Analytical Data
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基因名
HMGB1/HMG-1
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简介
The multifunctional and redox-sensitive HMGB1/HMG-1 protein plays multiple roles in cellular compartments. In the nucleus, it serves as a major chromatin-associated non-histone protein involved in key processes such as replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair, and genome stability. HMGB1/HMG-1 Protein, Human (HEK293, mFc) is the recombinant human-derived HMGB1/HMG-1 protein, expressed by HEK293, with C-mFc labeled tag.
- Application
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别名
rHuHigh mobility group protein B1/HMGB1, His; High Mobility Group Protein B1; High Mobility Group Protein 1; HMG-1; HMGB1; HMG1
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种属
Human
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表达系统
HEK293
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标签
C-mFc
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P09429
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表达区间
G2-E215
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蛋白长度
Partial
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分子量
55-65 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
High Mobility Group Box 1 (HMGB1), also known as HMG-1, is a non-histone chromatin-binding protein that plays a crucial role in various biological processes, including gene expression, DNA repair, and the modulation of the immune response. As a highly conserved protein, HMGB1 is present in almost all eukaryotic cells and is involved in the organization of nucleosomes, thereby influencing chromatin structure and function. Its significance extends beyond traditional roles in nuclear functions, as HMGB1 is also released extracellularly during cellular stress or injury, where it acts as a pro-inflammatory cytokine. Elevated levels of extracellular HMGB1 have been linked to various diseases, including cancer, sepsis, and autoimmune disorders, highlighting its potential as a therapeutic target and biomarker. The production of recombinant HMGB1/HMG-1 protein enables detailed studies of its structure-function relationships, post-translational modifications, and interactions with other biomolecules. Understanding these aspects is vital for elucidating HMGB1's dual roles in health and disease, ultimately guiding the development of novel therapeutic strategies aimed at modulating its activity. Researchers are particularly interested in investigating HMGB1's implications in cell signaling pathways, its mechanisms of action in inflammation, and its potential to serve as a target for immunotherapy, thus making HMGB1 a focal point in contemporary biomedical research.
