Analytical Data
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Gene name
HMGB1/HMG-1
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简介
The multifunctional and redox-sensitive HMGB1/HMG-1 protein plays multiple roles in cellular compartments. In the nucleus, it serves as a major chromatin-associated non-histone protein involved in key processes such as replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair, and genome stability. HMGB1/HMG-1 Protein, Human (HEK293, mFc) is the recombinant human-derived HMGB1/HMG-1 protein, expressed by HEK293, with C-mFc labeled tag.
- Application
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Alternative Names
rHuHigh mobility group protein B1/HMGB1, His; High Mobility Group Protein B1; High Mobility Group Protein 1; HMG-1; HMGB1; HMG1
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Species
Human
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Source
HEK293
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Tag
C-mFc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P09429
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Expression Region
G2-E215
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Protein Length
Partial
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Molecular Weight
55-65 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
High Mobility Group Box 1 (HMGB1), also known as HMG-1, is a non-histone chromatin-binding protein that plays a crucial role in various biological processes, including gene expression, DNA repair, and the modulation of the immune response. As a highly conserved protein, HMGB1 is present in almost all eukaryotic cells and is involved in the organization of nucleosomes, thereby influencing chromatin structure and function. Its significance extends beyond traditional roles in nuclear functions, as HMGB1 is also released extracellularly during cellular stress or injury, where it acts as a pro-inflammatory cytokine. Elevated levels of extracellular HMGB1 have been linked to various diseases, including cancer, sepsis, and autoimmune disorders, highlighting its potential as a therapeutic target and biomarker. The production of recombinant HMGB1/HMG-1 protein enables detailed studies of its structure-function relationships, post-translational modifications, and interactions with other biomolecules. Understanding these aspects is vital for elucidating HMGB1's dual roles in health and disease, ultimately guiding the development of novel therapeutic strategies aimed at modulating its activity. Researchers are particularly interested in investigating HMGB1's implications in cell signaling pathways, its mechanisms of action in inflammation, and its potential to serve as a target for immunotherapy, thus making HMGB1 a focal point in contemporary biomedical research.
