Analytical Data
-
基因名
EphB6
-
简介
EphB6 is a member of the receptor tyrosine kinase family expressed primarily in thymic cells and T cell subpopulations. EphB6, when stimulated by ephrin-B2, inhibited JNK activation, and decreased T-cell receptor-induced IL-2 secretion and CD25 expression. EphB6 promotes colorectal epithelial cell transformation and may serve as a novel biomarker and therapeutic target for colorectal cancer. EphB6 Protein, Cynomolgus (HEK293, Fc) is the recombinant cynomolgus-derived EphB6 protein, expressed by HEK293 , with C-hFc labeled tag.
- Application
-
生物活性
Measured by its binding ability in a functional ELISA. Immobilized Human EFNB2 at 10 μg/mL (100 μL/well) can bind Cynomolgus EphB6. The ED50 for this effect is 43.04 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized Human EFNB2 at 10 μg/ml (100 μL/well) can bind Cynomolgus EphB6. The ED50 for this effect is 43.04 ng/mL.
-
别名
Ephrin type-B receptor 6; HEP; Tyrosine-protein kinase-defective receptor EPH-6; EPHB6
-
种属
Cynomolgus
-
表达系统
HEK293
-
标签
C-hFc
-
纯度
Greater than 95% as determined by SDS-PAGE.
-
蛋白编号
A0A2K5X5C5
-
表达区间
L32-S590
-
氨基酸序列
LEEVLLDTTGETSEIGWLTYPPGGWDEVSVLDDQRRLTRTFEACHVAGAPPGTGQDNWLQTHFVERRGAQRAHIRLHFSVRACSSLGVSGGTCRETFTLYYRQAEEPDSPESVSSWHLKRWTKVDTIAADESFPSSSSSSSAAWAVGPHGAGQRAGLQLNVKERSFGPLTQRGFYVAFQDTGACLALVAVRLFSYTCPAVLRSFASFPETQASGAGGASLVAAVGTCVAHAEPEEDGVGGQAGGSPPRLHCNGEGKWMVAVGGCLCQPGHQPARGDKACQACPRGLYKASAGNVPCSPCPARSHAPNPAAPVCPCLEGFYRASSDPPEAPCTGPPSAPQELWFEVQGSALMLHWRLPRELGGRGDLLFNVVCKECEGRQEPASGGGGTCRRCRDEVHFDPRQRGLTESRVLVGGLRAHVPYILEVQAVNGVSELSPDPPQAAAINVSTSHEVPSAVPVVHQVSRASNSITVSWPQPDQTNGNILDYQLRYYDQAEDESHSFTLTSETNTATVTQLSPGHIYGFQVRARTAAGHGPYGGKVYFQTLPQGELSSQLPERLS
-
蛋白长度
Partial
-
分子量
90-110 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
EphB6 is a member of the Eph receptor tyrosine kinase family, which plays a crucial role in cell signaling processes governing developmental and physiological functions, particularly in the nervous system. Unlike other Eph receptors, EphB6 exhibits a unique characteristic of functioning mainly as a negative regulator of cell signaling pathways. This regulation is significant in various biological contexts, including cell proliferation, differentiation, and migration, particularly in neural tissues. Research has indicated that EphB6 may be implicated in tumor biology, as its expression levels might influence cancer progression and metastasis. In the context of neurodegenerative diseases, EphB6's role in modulating synaptic functions and neuronal interactions presents a potential therapeutic target. The recombinant production of EphB6 protein is essential for studying its biochemical properties, interactions with ligands, and downstream signaling mechanisms. Such studies can facilitate a deeper understanding of its biological roles and may pave the way for novel therapeutic strategies in treating diseases where EphB6's function is altered. Therefore, characterizing EphB6 in detail through recombinant protein technology is vital for elucidating its potential implications in health and disease.
