Analytical Data
-
Gene name
Vibrio cholerae serotype O1 CTXB 蛋白
-
简介
The pentameric ring of the B subunit of the CTXB protein guides the A subunit by binding to GM1 ganglioside on intestinal epithelial cells. This loop binds five GM1 gangliosides and promotes the specific interaction of the toxin with its cellular receptor. Vibrio cholerae serotype O1 CTXB Protein (HEK293, His) is the recombinant virus-derived Vibrio cholerae serotype O1 CTXB protein, expressed by HEK293, with C-His labeled tag.
- Application
-
Alternative Names
Cholera enterotoxin subunit B/CTxB (Vibrio cholerae); L(strain ATCC 39315 / El Tor Inaba N16961
-
Species
Virus
-
Source
HEK293
-
Tag
C-His
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P01556
-
Expression Region
T22-N124
-
Protein Length
Full Length of Mature Protein
-
Molecular Weight
16-17 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Vibrio cholerae serotype O1 is the bacterium responsible for cholera, a severe diarrheal disease that poses significant public health challenges worldwide, particularly in developing countries. The cholera toxin, primarily composed of the A and B subunits, plays a crucial role in the pathogenicity of V. cholerae, with the CTXB subunit being instrumental in the toxin's binding to intestinal epithelial cells. Research into the recombinant production of the CTXB protein is of great importance, as it facilitates the development of diagnostic tools and vaccines. By utilizing recombinant DNA technology, scientists can produce large quantities of CTXB, enabling studies on its structure, function, and interactions with host cells. This recombinant protein serves as a critical component in understanding cholera's molecular mechanisms, aiding in the design of effective immunological assays, and advancing vaccine candidates that may provide protective immunity against cholera. The ongoing research in this field not only contributes to our understanding of V. cholerae pathogenesis but also has the potential to significantly impact global health strategies aimed at controlling cholera outbreaks.
