Analytical Data
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Gene name
CDK6-CCND3 Heterodimer
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简介
The CDK6ic complex is an important serine/threonine protein kinase that regulates cell cycle progression and differentiation, promoting the G1/S transition by phosphorylating substrates such as pRB/RB1 and NPM1. It interacts with D-type G1 cyclins during interphase to form active pRB/RB1 kinase, which controls cell cycle entry. CDK6-CCND3 Heterodimer Protein, Human (sf9, GST, His) is a recombinant protein dimer complex containing human-derived CDK6-CCND3 Heterodimer protein, expressed by sf9 insect cells , with N-10*His, N-GST labeled tag.
- Application
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Alternative Names
CDK6; CCND3; Cyclin-dependent kinase 6; Cell division protein kinase 6; Serine/threonine-protein kinase PLSTIRE; G1/S-specific cyclin-D3
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Species
Human
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Source
Baculovirus
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Tag
N-GST;N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q00534 (M1-A326)&P30281
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Expression Region
Q00534 (M1-A326)&P30281 (M1-L292)
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The CDK6-CCND3 heterodimer is an essential complex involved in the regulation of the cell cycle and has gained significant attention in cancer research. Cyclin-dependent kinase 6 (CDK6) belongs to a family of serine/threonine kinases that, when associated with cyclins, orchestrate cell cycle progression from the G1 to S phase. Cyclin D3 (CCND3), a member of the cyclin D family, specifically partners with CDK6 to modulate its activity and stability, thereby influencing cell proliferation and differentiation. This heterodimer plays a pivotal role in cellular signaling pathways, affecting the transcription of genes vital for cell cycle progression. Dysregulation of the CDK6-CCND3 complex has been implicated in oncogenesis, with elevated levels observed in various tumors, suggesting its potential as a therapeutic target. The recombinant expression and characterization of the CDK6-CCND3 heterodimer are crucial for understanding its biochemical properties and functional roles. By producing this complex in vitro, researchers can elucidate its interactions with other regulatory proteins, investigate its kinase activity, and explore the impact of mutations associated with cancer. Consequently, this research not only enhances the understanding of cell cycle regulation but also paves the way for developing targeted therapies aimed at modulating the activity of the CDK6-CCND3 axis in cancer.
