Analytical Data
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Gene name
KPNB1
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简介
KPNB1 is a key player in nuclear protein import, cooperating with adapter proteins such as importin-alpha to recognize nuclear localization signals (NLS) in cargo substrates. In autonomous mode, it acts as its own NLS receptor, facilitating docking of the import/substrate complex to the nuclear pore complex (NPC) via KPNB1. KPNB1 Protein, Human (His) is the recombinant human-derived KPNB1 protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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Alternative Names
Importin subunit beta-1; Importin-90; Karyopherin subunit beta-1; Nuclear factor p97; Pore targeting complex 97 kDa subunit; PTAC97; KPNB1; NTF97
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q14974-1
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Expression Region
M1-A876
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AA Sequence
MELITILEKTVSPDRLELEAAQKFLERAAVENLPTFLVELSRVLANPGNSQVARVAAGLQIKNSLTSKDPDIKAQYQQRWLAIDANARREVKNYVLQTLGTETYRPSSASQCVAGIACAEIPVNQWPELIPQLVANVTNPNSTEHMKESTLEAIGYICQDIDPEQLQDKSNEILTAIIQGMRKEEPSNNVKLAATNALLNSLEFTKANFDKESERHFIMQVVCEATQCPDTRVRVAALQNLVKIMSLYYQYMETYMGPALFAITIEAMKSDIDEVALQGIEFWSNVCDEEMDLAIEASEAAEQGRPPEHTSKFYAKGALQYLVPILTQTLTKQDENDDDDDWNPCKAAGVCLMLLATCCEDDIVPHVLPFIKEHIKNPDWRYRDAAVMAFGCILEGPEPSQLKPLVIQAMPTLIELMKDPSVVVRDTAAWTVGRICELLPEAAINDVYLAPLLQCLIEGLSAEPRVASNVCWAFSSLAEAAYEAADVADDQEEPATYCLSSSFELIVQKLLETTDRPDGHQNNLRSSAYESLMEIVKNSAKDCYPAVQKTTLVIMERLQQVLQMESHIQSTSDRIQFNDLQSLLCATLQNVLRKVQHQDALQISDVVMASLLRMFQSTAGSGGVQEDALMAVSTLVEVLGGEFLKYMEAFKPFLGIGLKNYAEYQVCLAAVGLVGDLCRALQSNIIPFCDEVMQLLLENLGNENVHRSVKPQILSVFGDIALAIGGEFKKYLEVVLNTLQQASQAQVDKSDYDMVDYLNELRESCLEAYTGIVQGLKGDQENVHPDVMLVQPRVEFILSFIDHIAGDEDHTDGVVACAAGLIGDLCTAFGKDVLKLVEARPMIHELLTEGRRSKTNKAKTLATWATKELRKLKNQA
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Protein Length
Full Length of Isoform-1
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Molecular Weight
90 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
KPNB1, or importin beta 1, is a crucial protein involved in the nuclear transport pathway, specifically functioning as a karyopherin that facilitates the translocation of proteins into the nucleus. Its role in regulating nuclear-cytoplasmic transport has made KPNB1 a significant focus of research, particularly in the context of cellular processes such as gene expression, cell cycle regulation, and response to stress. Abnormalities in nuclear transport mechanisms, including those involving KPNB1, have been implicated in various diseases, including cancer, neurodegenerative disorders, and viral infections. The study of KPNB1 is further propelled by its interactions with a range of proteins, influencing cellular signaling pathways and impacting cellular localization. Recombinant KPNB1 protein is often produced for experimental applications, allowing researchers to investigate its structure, function, and interactions in detail. By understanding KPNB1's mechanisms in healthy and diseased states, scientists aim to uncover potential therapeutic targets that could be instrumental in developing novel treatments for diseases associated with impaired nuclear transport. Overall, KPNB1 serves as a key player in cellular homeostasis, and the characterization of its recombinant protein can provide valuable insights into the fundamental processes of cell biology and pathology.
