Analytical Data
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Gene name
UbcH7/UBE2L3
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简介
UbcH7/UBE2L3 is a unique ubiquitin-conjugating E2 enzyme that cooperates with HECT-type and RBR family E3 ligases and lacks intrinsic E3-independent reactivity with lysine. It uniquely works with RBR family E3 enzymes such as PRKN, RNF31 and ARIH1, acting like a RING-HECT hybrid. UbcH7/UBE2L3 Protein, Human (sf9, His, Strep) is the recombinant human-derived UbcH7/UBE2L3 protein, expressed by sf9 insect cells , with N-Strep, N-8*His labeled tag.
- Application
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Alternative Names
UBE2L3; Ubiquitin-conjugating enzyme E2 L3; E2 ubiquitin-conjugating enzyme L3; L-UBC; UbcH7; Ubiquitin carrier protein L3; Ubiquitin-conjugating enzyme E2-F1; Ubiquitin-protein ligase L3
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Species
Human
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Source
Baculovirus
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Tag
N-StrepⅡ;N-8*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P68036
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Expression Region
A2-D154
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Protein Length
Partial
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
UbcH7, also known as UBE2L3, is an E2 ubiquitin-conjugating enzyme that plays a critical role in the ubiquitin-proteasome system, which is essential for regulating protein degradation, cellular signaling, and various biological processes. The research on UbcH7 has gained prominence due to its involvement in diverse cellular pathways and its potential implications in various diseases, including cancer. UbcH7 is known to interact with various E3 ligases, mediating the transfer of ubiquitin moieties to target substrates, thus influencing their stability and function. Dysregulation of UbcH7 activity has been implicated in tumorigenesis and other pathologies, making it a focal point for therapeutic investigations. Moreover, recombinant UbcH7 protein serves as a valuable tool for elucidating the mechanisms of ubiquitination and its effects on cellular homeostasis. The production and characterization of this protein can facilitate in-depth studies of its enzymatic properties, substrate specificity, and interactions with E3 ligases, thereby contributing to our understanding of the ubiquitin system as a whole. Researchers aim to explore the structural and functional aspects of UbcH7 to potentially identify novel targets for drug development, highlighting the importance of this enzyme in both fundamental biology and medicinal research.
