Analytical Data
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基因名
UbcH6/UBE2E1
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简介
The UbcH6/UBE2E1 protein is an important ubiquitination component that acts as an E2 ubiquitin-conjugating enzyme, accepting ubiquitin in the E1 complex and catalyzing its covalent attachment to proteins. It is versatile and also facilitates covalent attachment of ISG15, playing a role in a variety of ubiquitin-like modifications. UbcH6/UBE2E1 Protein, Human (sf9, His, Strep) is the recombinant human-derived UbcH6/UBE2E1 protein, expressed by sf9 insect cells , with N-Strep, N-8*His labeled tag.
- Application
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别名
UBE2E1; Ubiquitin-conjugating enzyme E2 E1; (E3-independent) E2 ubiquitin-conjugating enzyme E1; E2 ubiquitin-conjugating enzyme E1; UbcH6; Ubiquitin carrier protein E1; Ubiquitin-protein ligase E1
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种属
Human
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表达系统
Baculovirus
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标签
N-StrepⅡ;N-8*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P51965
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表达区间
S2-T193
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蛋白长度
Full Length of Mature Protein
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
UbcH6, also known as UBE2E1, is a member of the ubiquitin-conjugating enzyme family that plays a crucial role in the ubiquitin-proteasome system, responsible for protein degradation and various cellular regulatory processes. The ubiquitylation process, mediated by UbcH6, involves the attachment of ubiquitin molecules to target proteins, which can signal for their degradation, alter their cellular location, or modify their activity. Research on UbcH6 has gained significant attention due to its involvement in various biological pathways linked to cell cycle regulation, DNA repair, and responses to stress. Consequently, dysregulation of UbcH6 expression or activity has been implicated in numerous diseases, including cancer and neurodegenerative disorders. The study of recombinant UbcH6/UBE2E1 aims to provide insights into its structural and functional characteristics, facilitating a deeper understanding of its role in ubiquitination and potential therapeutic applications. By expression and purification of the recombinant protein, researchers can investigate its enzyme kinetics, substrate specificity, and interactions with other proteins, contributing to the broader understanding of the ubiquitin-proteasome system's role in cellular homeostasis and disease pathology.
