Analytical Data
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Gene name
CK2 alpha/CSNK2A1
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简介
CK2α/CSNK2A1 protein can phosphorylate serine or threonine. It is involved in the regulation of cell cycle, apoptosis, transcription and viral infection, and has protective effects on the spindle assembly checkpoint and p53/TP53-mediated apoptosis. CK2α/CSNK2A1 can also participate in signal transduction by phosphorylating various factors, affecting circadian clock function and virus life cycle. CK2 alpha/CSNK2A1 Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived CK2 alpha/CSNK2A1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
- Application
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Alternative Names
Casein kinase II subunit alpha; CK II alpha; CSNK2A1; CK2A1
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Species
Mouse
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Source
Baculovirus
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Tag
N-His;N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q60737
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Expression Region
M1-Q391
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Protein Length
Full Length
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Molecular Weight
65 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CK2 alpha, also known as CSNK2A1, is a pivotal serine/threonine kinase involved in various cellular processes, including cell proliferation, survival, and differentiation. This enzyme operates as a tetrameric protein complex, consisting of two catalytic subunits (alpha) and two regulatory subunits (beta), enabling its diverse regulatory functions. The dysregulation of CK2 alpha activity has been implicated in several malignancies, highlighting its potential as a therapeutic target in cancer treatment. Research has recently focused on the generation of recombinant CK2 alpha proteins to facilitate the study of its biochemical properties, substrate specificity, and interaction with other cellular proteins. By utilizing techniques such as molecular cloning and protein expression systems, researchers can obtain high-purity CK2 alpha for in vitro assays and drug screening. Understanding the structure-function relationship of CK2 alpha through recombinant protein studies could elucidate its role in oncogenesis and lead to the development of specific inhibitors, enhancing the therapeutic arsenal against CK2-related cancers. Furthermore, KS2A1's involvement in other cellular pathways, such as DNA damage response and circadian rhythms, underscores the importance of these studies in contributing to a broader understanding of CK2's biological significance.
