Recombinant Human MD-2/LY96 Protein，His

MD-2/LY96

MD2 protein binds bacterial lipopolysaccharide (LPS) and collaborates with TLR4 in the innate immune response to LPS. It also interacts with TLR2 in the response to cell wall components from bacteria. MD2 enhances TLR4-dependent NF-kappa-B activation and forms a heterogeneous homomer, participating in a multi-protein complex with CD14 and TLR4. Ligand binding induces complex oligomerization, crucial for the cellular response to LPS. MD-2/LY96 Protein, Human (142a.a, His) is the recombinant human-derived MD2 protein, expressed by E. coli , with C-His labeled tag.

SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

Immobilized Human TLR-4 at 2 μg/mL (100 μL/well) can bind Biotinylated Human MD2. The ED50 for this effect is 0.08207 μg/mL. Immobilized Human TLR-4 at 2 μg/mL (100 μL/well) can bind Biotinylated Human MD2. The ED50 for this effect is 0.08207 μg/mL.

Lymphocyte antigen 96; LY96; Ly-96; ESOP1; ESOP-1; MD-2;

Human

E. coli

C-8*His

Greater than 90% as determined by SDS-PAGE.

Q9Y6Y9-1

Q19-N160

Full Length of Isoform-1 Mature Protein

18 kDa

< 1.0 EU per μg protein as determined by the LAL method.

Freeze-dried powder

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.