Analytical Data
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Gene name
AK3L1
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简介
The AK3L1 protein actively maintains cellular nucleotide homeostasis by catalyzing the interconversion of nucleoside phosphates. This multifunctional enzyme efficiently phosphorylates AMP and dAMP using ATP as a phosphate donor, and selectively phosphorylates AMP when utilizing GTP. AK3L1 Protein, Human (sf9) is the recombinant human-derived AK3L1 protein, expressed by Sf9 insect cells , with tag free.
- Application
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Alternative Names
Adenylate kinase 4; Adenylate kinase 3-like; GTP:AMP phosphotransferase AK4; AK3
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Species
Human
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Source
Baculovirus
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Tag
Tag Free
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P27144
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Expression Region
A2-Y223
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Protein Length
Partial
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Molecular Weight
27 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
AK3L1 (Adenylate Kinase 3-Like 1) is a member of the adenylate kinase family, which plays a crucial role in cellular energy homeostasis by catalyzing the conversion of ADP to ATP and AMP. This enzyme is particularly interesting due to its potential involvement in various cellular processes, including apoptosis, cell proliferation, and stress responses. Research has shown that AK3L1 is widely expressed in multiple tissues, indicating its fundamental role in energy metabolism across different organ systems. Abnormal expression or dysfunction of AK3L1 may be implicated in a variety of pathological conditions, including metabolic disorders and cancer. Therefore, understanding the functional properties and regulatory mechanisms of AK3L1 is vital for elucidating its role in health and disease. Studies focusing on the recombinant expression and purification of AK3L1 protein are essential to explore its biochemical characteristics, substrate specificity, and interaction with other cellular components. By investigating the structure-function relationship of AK3L1, researchers aim to uncover its precise biological roles, providing insights that could lead to novel therapeutic strategies targeting energy metabolism-related diseases. The development of recombinant AK3L1 also paves the way for high-throughput screening of potential inhibitors or modulators, enhancing our understanding of its functional significance in various cellular contexts. Overall, the research on AK3L1 recombinant protein holds promise for advancing our knowledge of metabolic regulation and the potential development of targeted interventions in diseases linked to energy imbalance.
