Analytical Data
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Gene name
NODAL
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简介
Nodal protein plays a key role in embryonic development and is indispensable for mesoderm formation and axial patterning. As a homodimer linked together by disulfide bonds, Nodal helps form the molecular framework that controls fundamental developmental pathways, ensuring the correct establishment of mesodermal tissue and axial structure during embryogenesis. NODAL Protein, Human is the recombinant human-derived NODAL protein, expressed by E. coli, with tag free.
- Application
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Alternative Names
HTX5; NODAL; Nodal homolog
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Species
Human
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96S42
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Expression Region
H238-L347
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Protein Length
Full Length of Mature Protein
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Molecular Weight
14-15 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NODAL is a member of the TGF-beta superfamily of secreted signaling proteins that plays a crucial role in early embryonic development and the establishment of the body plan in vertebrates. It is primarily involved in regulating processes such as mesoderm formation, endoderm specification, and germ layer patterning. The study of NODAL has gained significant interest due to its pivotal functions in developmental biology, including the induction of pluripotency and maintenance of stem cell characteristics. Dysregulation of NODAL signaling has been implicated in various developmental disorders and diseases, including cancer, highlighting its potential as a therapeutic target. Research has focused on the mechanisms of NODAL signaling pathways, its interactions with other morphogens, and the effects of NODAL on cellular behaviors such as proliferation, differentiation, and migration. Additionally, studies have explored the role of NODAL in tissue regeneration and its impact on the microenvironment of stem cells. Overall, the exploration of NODAL's functions and regulatory mechanisms continues to provide valuable insights into both developmental processes and the underlying causes of various pathologies.
