Analytical Data
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Gene name
PTPRC/CD45RABC
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简介
Receptor-type tyrosine-protein phosphatase C (PTPRC) is a receptor type PTP and an essential positive regulator of T- and B-cell antigen receptor signaling. PTPRC functions through direct interaction with antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. PTPRC also suppresses JAK kinases as a regulator of cytokine receptor signaling. PTPRC/CD45RABC Protein, Human (HEK293, hFc) is the recombinant human-derived PTPRC/CD45RABC protein, expressed by HEK293 , with C-hFc labeled tag.
- Application
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Alternative Names
L-CA; CD45; T200; PTPRC; B220; CD45R; GP180; LCA; LY5; EC 3.1.3.48
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Species
Human
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Source
HEK293
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Tag
C-hFc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P08575-3
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Expression Region
Q26-K577
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Protein Length
Extracellular Domain
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Molecular Weight
120-160 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PTPRC, also known as CD45, is a crucial protein tyrosine phosphatase that plays a significant role in regulating cellular signaling in immune cells, particularly T and B lymphocytes. The CD45 molecule is complex, existing in several isoforms that arise from alternative splicing of its transcripts, notably CD45RA, CD45RO, and CD45RABC, each with distinct functions in immune responses and cell activation. CD45RABC, which represents the most universally expressed isoform in lymphocytes, is pivotal for modulating T cell receptor signaling and lymphocyte activation. Dysregulation of CD45 expression is implicated in various autoimmune diseases and cancers, making it a critical target for therapeutic intervention. Research on recombinant PTPRC/CD45RABC proteins has advanced our understanding of its structural and functional characteristics, allowing for insights into its signaling mechanisms. Moreover, the generation of recombinant CD45RABC proteins facilitates the exploration of potential roles in immune therapies and diagnostics, paving the way for novel immunomodulatory strategies. Studies utilizing recombinant forms have highlighted the importance of post-translational modifications and interactions with other signaling molecules that influence the immune response. As a result, ongoing research aims to leverage these insights to develop targeted treatments that can restore normal immune function in pathological conditions. Understanding the precise functions of CD45RABC and its isoforms continues to be a pivotal area of immunological research, potentially leading to breakthroughs in clinical applications.
