Analytical Data
-
基因名
GPT2
- Application
-
别名
(ALT2)(Glutamate pyruvate transaminase 2)(GPT 2)(Glutamic--alanine transaminase 2)(Glutamic--pyruvic transaminase 2)
-
种属
Human
-
表达系统
E. coli
-
标签
N- His & C- Myc
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
Q8TD30
-
表达区间
1-523aa
-
分子量
65.3 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of GPT2 (Glutamate Pyruvate Transaminase 2) recombinant protein is rooted in its crucial role in amino acid metabolism, particularly in the transamination process between glutamate and pyruvate, which is essential for regulating glucose and energy homeostasis in cells. GPT2 is predominantly expressed in the liver, where it contributes to gluconeogenesis and nitrogen metabolism. Given the increasing incidence of metabolic disorders, such as obesity and diabetes, there is a growing interest in understanding the regulatory mechanisms of enzymes like GPT2. The production of recombinant GPT2 allows researchers to obtain large quantities of the protein for detailed biochemical analysis, structural studies, and enzyme kinetics, facilitating the exploration of its cellular functions and interactions. Furthermore, generating GPT2 in a recombinant system enables the study of site-specific mutations, which can elucidate the enzyme's functional domains and regulatory pathways. As such, research on recombinant GPT2 not only enhances our understanding of basic metabolic processes but also opens avenues for potential therapeutic interventions targeting metabolic diseases, highlighting the significance of this protein in both health and disease contexts.
