Analytical Data
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Gene name
TNF-alpha/TNFSF2
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简介
TNF-α/TNFSF2 protein is secreted by macrophages, binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR, induces tumor cell death and acts as a pyrogen.It is associated with cachexia, stimulates cell proliferation and differentiation, and induces insulin resistance in adipocytes, leading to TNF-induced insulin resistance.TNF-alpha/TNFSF2 Protein, Mouse (His-SUMO) is the recombinant mouse-derived TNF-alpha/TNFSF2 protein, expressed by E.coli , with N-SUMO, N-6*His labeled tag.
- Application
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Alternative Names
rHuTNF-α, His; Cachectin; TNFSF2
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Species
Mouse
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Source
E. coli
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Tag
N-SUMO;N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P06804
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Expression Region
G57-L235
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Protein Length
Extracellular Domain
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Molecular Weight
35-37 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Tumor Necrosis Factor-alpha (TNF-alpha), also known as TNFSF2, is a pivotal pro-inflammatory cytokine involved in various physiological and pathological processes, including immune response, cell proliferation, apoptosis, and inflammation. Its dysregulation is implicated in several diseases, such as autoimmune disorders, cancer, and chronic inflammatory conditions. Understanding the biological mechanisms of TNF-alpha has drawn considerable interest within the scientific community, leading to the development of therapeutic agents targeting its signaling pathways. Recombinant TNF-alpha proteins have been produced for research purposes to study their roles in cell signaling and as potential therapeutic targets. These recombinant proteins enable researchers to investigate the functional variations of TNF-alpha, its receptor interactions, and the downstream signaling pathways it activates. Furthermore, they facilitate the assessment of pharmacological interventions aimed at modulating TNF-alpha activity. The exploration of TNF-alpha's role in disease contexts has underscored the significance of this cytokine in medical research, leading to the development of TNF inhibitors as effective treatments for inflammatory diseases. Overall, the study of TNF-alpha/TNFSF2 recombinant proteins is critical for unveiling novel insights into immune regulation and developing targeted therapies for a range of inflammatory conditions.
