Analytical Data
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Gene name
TGF alpha/TGFA
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简介
TGF α/TGFA is a mitogenic peptide that binds to the EGF receptor (EGFR) and acts synergistically with TGF β to achieve anchorage-independent cell proliferation in soft agar.It interacts with SDCBP and the SNTA1 PDZ domain, which is critical for cell surface localization.TGF alpha/TGFA Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived TGF alpha/TGFA protein, expressed by HEK293 , with N-hFc labeled tag.
- Application
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Biological Activity
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is <0.4 ng/mL. Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.2054 ng/mL, corresponding to a specific activity is 4.869×106 units/mg.
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Alternative Names
ETGF; TGF-alpha; TGF type 1; TGFA
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Species
Mouse
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Source
HEK293
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Tag
N-hFc
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Purity
Greater than 95% as determined by reducing SDS-PAGE.
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Uniprot
P48030
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Expression Region
V39-A88
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Protein Length
Partial
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Molecular Weight
38-42 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Transforming Growth Factor Alpha (TGF-alpha), also known as TGFA, is a key member of the EGF (Epidermal Growth Factor) family of peptides, which plays a critical role in cellular proliferation, differentiation, and survival. This protein is predominantly involved in the regulation of epithelial development and tissue repair, making it crucial in various physiological and pathological processes, including wound healing, organ regeneration, and tumorigenesis. Research has shown that TGF-alpha is overexpressed in several types of cancers, contributing to increased cell growth and metastasis. Consequently, understanding the molecular mechanisms underlying TGF-alpha signaling can provide valuable insights for therapeutic interventions in cancer and other diseases. The development of recombinant TGF-alpha proteins has enabled scientists to study its biological functions in greater detail, allowing for the exploration of its potential as a therapeutic target. By utilizing advanced recombinant DNA technology, researchers can produce large quantities of functional TGF-alpha, facilitating studies on its interactions with specific receptors and downstream signaling pathways. Furthermore, recombinant TGF-alpha can be employed in various in vitro and in vivo models to elucidate its effects on cellular behaviors and to test novel cancer treatments. Overall, the research surrounding TGF-alpha/TGFA recombinant proteins is not only essential for enhancing our understanding of growth factor biology but also holds promise for innovative therapeutic strategies in oncology and regenerative medicine.
