Analytical Data
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基因名
GPT
- Application
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别名
GPT; ALAT; SGPT; AAT1; GPT1; Serum Glutamic Pyruvic Transaminase; Alanine Transaminase; Glutamate pyruvate transaminase 1; Glutamic--alanine transaminase 1
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种属
Human
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表达系统
E. coli
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标签
N-His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
P24298
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表达区间
Ile147~Phe263
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分子量
14kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Research on GPT (glutamate pyruvate transaminase) recombinant proteins has gained significant attention due to their crucial role in metabolic pathways and potential therapeutic applications. GPT, an enzyme primarily found in the liver and kidneys, catalyzes the reversible transfer of amino groups between glutamate and pyruvate, leading to the production of α-ketoglutarate and alanine. This reaction is vital for amino acid metabolism and energy homeostasis. The recombinant production of GPT proteins allows for detailed biochemical studies, facilitating the exploration of their structure-function relationships and enzymatic mechanisms. Moreover, the ability to produce these proteins in a controlled manner enables researchers to investigate their roles in various diseases, particularly metabolic disorders and liver dysfunctions. As the understanding of GPT's involvement in pathological conditions grows, there is increasing interest in leveraging recombinant GPT proteins for diagnostic and therapeutic strategies. This includes the potential development of novel drugs targeting metabolic pathways influenced by GPT activity, as well as the use of GPT as a biomarker for assessing liver function. The advancement of genetic engineering and protein expression systems has further propelled research into GPT recombinant proteins, making them invaluable tools in both basic and applied biomedical research.












