Analytical Data
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基因名
Stratifin
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简介
Stratifin (14-3-3 sigma), encoded by SFN, functions as a multifaceted adapter protein that binds to partners through phosphoserine or phosphothreonine motifs and participates in a variety of cellular processes. It regulates epithelial cell growth and protein synthesis through keratin 17 (KRT17), and may affect MDM2 autoubiquitination and activate p53. Stratifin Protein, Human (N-His, C-Myc) is the recombinant human-derived Stratifin protein, expressed by E. coli , with N-10*His, C-Myc labeled tag.
- Application
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别名
14-3-3 Protein Sigma; Epithelial Cell Marker Protein 1; Stratifin; SFN; HME1
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种属
Human
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表达系统
E. coli
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标签
N-10*His;C-Myc
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P31947-1
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表达区间
M1-S248
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氨基酸序列
MERASLIQKAKLAEQAERYEDMAAFMKGAVEKGEELSCEERNLLSVAYKNVVGGQRAAWRVLSSIEQKSNEEGSEEKGPEVREYREKVETELQGVCDTVLGLLDSHLIKEAGDAESRVFYLKMKGDYYRYLAEVATGDDKKRIIDSARSAYQEAMDISKKEMPPTNPIRLGLALNFSVFHYEIANSPEEAISLAKTTFDEAMADLHTLSEDSYKDSTLIMQLLRDNLTLWTADNAGEEGGEAPQEPQS
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蛋白长度
Full Length of Isoform-1
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分子量
34 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Stratifin, also known as SFN or 14-3-3 sigma, is a member of the 14-3-3 protein family that plays a crucial role in various cellular processes, including cell cycle regulation, apoptosis, and stress response. Research on Stratifin has gained prominence due to its significant implications in cancer biology and its potential as a biomarker for various malignancies. In normal tissues, Stratifin is involved in the regulation of cell proliferation and differentiation, acting as a tumor suppressor. However, its expression is often downregulated or lost in several types of tumors, contributing to uncontrolled cell growth and tumor progression. Understanding the mechanisms governing Stratifin's function and its interactions with other molecular pathways is essential for elucidating its role in tumorigenesis. Recent studies have focused on the recombinant production of Stratifin to investigate its structural and functional properties, providing insights into its biological activity. These recombinant proteins enable detailed studies of Stratifin's interactions with other cellular proteins, offering potential avenues for therapeutic intervention in cancer treatment. The exploration of Stratifin's role in cellular signaling and its potential use as a therapeutic target highlights the need for continued research in this area, which could pave the way for novel approaches in cancer diagnostics and treatment strategies.
