Analytical Data
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Gene name
Afamin/AFM
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简介
Afamin/AFM protein serves as a carrier of hydrophobic molecules in body fluids and plays an important role in the solubility and activity of lipidated Wnt family members (including WNT1, WNT2B, WNT3, WNT3A, WNT5A, WNT7A, WNT7B, WNT8, WNT9A, and WNT9B). The role of WNT10A and WNT10B. It also binds to vitamin E and may act as a transporter of this vitamin in body fluids, especially when the lipoprotein system is deficient. Afamin/AFM Protein, Human (578a.a, HEK293, His) is the recombinant human-derived Afamin/AFM protein, expressed by HEK293 , with C-10*His labeled tag.
- Application
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Biological Activity
Measured by its ability to chemoattract bioassay using MC3T3-E1 cells. The ED50 for this effect is 15.01ng/mL, corresponding to a specific activity is 4.657×104 units/mg. Measured by its ability to chemoattract bioassay using MC3T3-E1 cells.The ED50 for this effect is 15.01 ng/mL， corresponding to a specific activity is 4.657×104 units/mg.
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Alternative Names
rHuAfamin, His; Afamin; AFM; ALB2; ALB2alpha-Alb; ALBA; ALBAalpha-albumin; ALF; Alpha-Alb; Alpha-albumin
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Species
Human
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Source
HEK293
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Tag
C-10*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
P43652
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Expression Region
L13-N599
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AA Sequence
LFFLTESLTLPTQPRDIENFNSTQKFIEDNIEYITIIAFAQYVQEATFEEMEKLVKDMVEYKDRCMADKTLPECSKLPNNVLQEKICAMEGLPQKHNFSHCCSKVDAQRRLCFFYNKKSDVGFLPPFPTLDPEEKCQAYESNRESLLNHFLYEVARRNPFVFAPTLLTVAVHFEEVAKSCCEEQNKVNCLQTRAIPVTQYLKAFSSYQKHVCGALLKFGTKVVHFIYIAILSQKFPKIEFKELISLVEDVSSNYDGCCEGDVVQCIRDTSKVMNHICSKQDSISSKIKECCEKKIPERGQCIINSNKDDRPKDLSLREGKFTDSENVCQERDADPDTFFAKFTFEYSRRHPDLSIPELLRIVQIYKDLLRNCCNTENPPGCYRYAEDKFNETTEKSLKMVQQECKHFQNLGKDGLKYHYLIRLTKIAPQLSTEELVSLGEKMVTAFTTCCTLSEEFACVDNLADLVFGELCGVNENRTINPAVDHCCKTNFAFRRPCFESLKADKTYVPPPFSQDLFTFHADMCQSQNEELQRKTDRFLVNLVKLKHELTDEELQSLFTNFANVVDKCCKAESPEVCFNEESPKIGN
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Protein Length
Partial
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Molecular Weight
80-130 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Afamin (AFM) is a member of the albumin family of proteins, primarily found in the bloodstream where it plays a crucial role in transporting vitamin E and other lipophilic molecules. Research on Afamin has gained traction due to its potential implications in various physiological and pathological processes, including cardiovascular health, metabolic disorders, and even cancer. Recent studies have shown that Afamin levels may serve as biomarkers for certain diseases, and its dysregulation is associated with conditions such as diabetes and atherosclerosis. The recombinant production of Afamin protein offers a promising avenue for exploring its biological functions and the mechanisms underlying its association with disease states. By utilizing recombinant DNA technology, researchers can generate large quantities of Afamin to study its structure, function, and interactions with other molecules in detail. This has paved the way for potential therapeutic applications, where recombinant Afamin could be used in treatments that target vitamin E deficiency or related metabolic dysfunctions. Furthermore, understanding the precise role of Afamin can contribute to the development of novel diagnostic tools, enhancing our ability to predict and monitor disease progression. Overall, ongoing research into recombinant Afamin not only deepens our understanding of this protein's significance but also highlights its potential as a biomarker and therapeutic agent in modern medicine.
