Analytical Data
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基因名
GPX4
- Application
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生物活性
GXP4 activity is measured indirectly. GXP4 converts reduced glutathione (GSH) to oxidized glutathione (GSSG) by reducing hydroperoxide (ROOH). GSSG is then converted back to its reduced form by glutathione reductase (GR) in the presence of NAPDH, which is converted into NADP+. NADPH is intrinsically fluorescent, a property lost upon oxidation to NADP+. The fluorescence signal is inversely proportional to the enzyme concentration. This is because with high GPX4 concentrations, you have more NADPH (fluorescent molecule) converting to NADP+.
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别名
Phospholipid hydroperoxide glutathione peroxidase; PHGPx; Glutathione peroxidase 4; GPx-4; GSHPx-4
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种属
Human
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表达系统
HEK293
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标签
N-Flag
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P36969-1
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表达区间
C29-F197, U73C
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蛋白长度
Full Length of Isoform-1 Mature Protein
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分子量
21 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GPX4 (Glutathione Peroxidase 4) is a selenoprotein that plays a crucial role in protecting cells from oxidative stress by reducing lipid hydroperoxides into their corresponding alcohols, thereby maintaining cellular redox homeostasis. The research on GPX4 has gained significant attention due to its involvement in various biological processes, including ferroptosis, a form of regulated cell death characterized by the accumulation of lipid peroxides. Dysregulation of GPX4 has been implicated in several diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases. Recent studies have highlighted the potential of GPX4 as a therapeutic target for promoting cell survival under oxidative stress conditions and for developing novel treatments against ferroptosis-related pathologies. Understanding the structure and function of GPX4, along with the mechanisms regulating its expression and activity, is essential for elucidating its role in cellular protection and disease progression. The recombinant production of GPX4 allows for detailed biochemical and biophysical characterization, aiding in the investigation of its antioxidant functions and interactions with other cellular components. Overall, research on GPX4 is pivotal in advancing our knowledge of oxidative stress management and developing strategies for the prevention and treatment of oxidative damage-related diseases.
