Recombinant Human GPX4 Protein (HEK293)，Flag

GPX4

SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

GXP4 activity is measured indirectly. GXP4 converts reduced glutathione (GSH) to oxidized glutathione (GSSG) by reducing hydroperoxide (ROOH). GSSG is then converted back to its reduced form by glutathione reductase (GR) in the presence of NAPDH, which is converted into NADP+. NADPH is intrinsically fluorescent, a property lost upon oxidation to NADP+. The fluorescence signal is inversely proportional to the enzyme concentration. This is because with high GPX4 concentrations, you have more NADPH (fluorescent molecule) converting to NADP+.

Phospholipid hydroperoxide glutathione peroxidase; PHGPx; Glutathione peroxidase 4; GPx-4; GSHPx-4

Human

HEK293

N-Flag

Greater than 90% as determined by SDS-PAGE.

P36969-1

C29-F197, U73C

Full Length of Isoform-1 Mature Protein

21 kDa

< 1.0 EU per μg protein as determined by the LAL method.

Freeze-dried powder

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.