Analytical Data
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基因名
CCND1
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简介
The CCND1 protein is a regulatory component of the cyclin D1-CDK4 complex that coordinates phosphorylation and RB family inhibition to regulate the G(1)/S transition. This promotes the dissociation of E2F from the RB/E2F complex and promotes the transcription of E2F target genes critical for G(1) phase progression. CCND1 Protein, Human (His) is the recombinant human-derived CCND1 protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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种属
Human
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表达系统
E. coli
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标签
N-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P24385
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表达区间
M1-I295
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氨基酸序列
MEHQLLCCEVETIRRAYPDANLLNDRVLRAMLKAEETCAPSVSYFKCVQKEVLPSMRKIVATWMLEVCEEQKCEEEVFPLAMNYLDRFLSLEPVKKSRLQLLGATCMFVASKMKETIPLTAEKLCIYTDNSIRPEELLQMELLLVNKLKWNLAAMTPHDFIEHFLSKMPEAEENKQIIRKHAQTFVALCATDVKFISNPPSMVAAGSVVAAVQGLNLRSPNNFLSYYRLTRFLSRVIKCDPDCLRACQEQIEALLESSLRQAQQNMDPKAAEEEEEEEEEVDLACTPTDVRDVDI
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蛋白长度
Full Length
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分子量
37 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Cyclin D1 (CCND1) is a key regulatory protein involved in cell cycle progression, particularly in the transition from the G1 phase to the S phase. Abnormal expression or mutation of CCND1 has been associated with various cancers, including breast, lymphoma, and colorectal cancer, making it a significant focus in oncological research. The gene encoding CCND1 is often subjected to genetic alterations such as amplifications and translocations, which can lead to elevated protein levels and aberrant cell proliferation. Given its pivotal role in cellular proliferation and tumorigenesis, the study of CCND1 recombinant protein has garnered attention for both its potential as a biomarker and its utility in therapeutic approaches. Researchers have been investigating the structural and functional characteristics of the CCND1 protein to understand its interactions with cyclin-dependent kinases (CDKs) and other regulatory proteins within the cell cycle machinery. By producing recombinant CCND1, scientists can explore its biochemical properties, develop specific inhibitors, and assess its role in various signaling pathways, thereby paving the way for targeted cancer therapies. Additionally, studying CCND1's interaction with other cellular components could elucidate mechanisms of resistance to existing treatments, ultimately improving therapeutic regimens for cancer patients. Overall, the investigation of CCND1 recombinant protein serves as a crucial step toward unraveling the complexities of tumor biology and enhancing cancer treatment strategies.
