Analytical Data
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Gene name
colE7
- Application
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Alternative Names
Colicin-E7; cea; colE7
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Species
E.coli
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Source
E. coli
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Tag
C-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q47112
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Expression Region
M1-K576
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Protein Length
Full Length
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ColE7 is a plasmid-borne antibiotic produced by the bacterium *E. coli*, which exhibits significant antibacterial activity against various Gram-negative and Gram-positive pathogens. The research surrounding colE7 recombinant protein has gained momentum due to the urgent need for novel antimicrobial agents in the face of rising antibiotic resistance. ColE7 is part of a broader class of proteins known as colicins, which are bacteriocins that display specific killing mechanisms targeting rival bacterial strains. Understanding the structure, function, and mode of action of colE7 is crucial for its potential application in therapeutics and biocontrol strategies. Scientists have focused on the expression and purification of colE7 to study its activity, stability, and interaction with bacterial membranes. Additionally, the ability to genetically engineer and modify colE7 opens pathways for enhancing its antimicrobial properties and expanding its spectrum of activity. Research on colE7 not only contributes to basic microbiological knowledge but also has significant implications for developing alternative treatments to combat resistant infections, highlighting its potential role in modern medicine and biotechnology.
