Analytical Data
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基因名
BRPF1
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简介
The BRPF1 protein is a key scaffold in HAT complexes such as MOZ/MORF and HBO1, and has histone H3 acetyltransferase activity. In the HBO1 complex, BRPF1 directs the specificity of KAT7/HBO1 for H3K14ac. BRPF1 Protein, Human (His) is the recombinant human-derived BRPF1 protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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别名
BRPF1; Peregrin; Bromodomain and PHD finger-containing protein 1; Protein Br140
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种属
Human
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表达系统
E. coli
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标签
N-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P55201
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表达区间
E1079-S1207
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蛋白长度
Partial
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
BRPF1 (Bromodomain and PHD Finger Containing 1) is a protein that plays a crucial role in chromatin remodeling and gene regulation, impacting various biological processes, including cell proliferation and differentiation. Emerging research has illuminated its involvement in multiple diseases, particularly cancers, where aberrations in its expression or function can lead to dysregulated cell growth. The study of BRPF1 is particularly relevant given its unique structural features, including bromodomains that recognize acetylated lysines on histones, which are vital for the epigenetic control of gene expression. Moreover, BRPF1 is a component of several protein complexes that facilitate transcriptional activation and chromatin modification, underscoring its significance in maintaining cellular homeostasis. Investigating BRPF1 as a recombinant protein allows researchers to explore its functional properties in detail, including binding affinities and interactions with other chromatin-associated proteins. Understanding the molecular mechanisms by which BRPF1 regulates gene expression could reveal novel therapeutic targets, especially in cancer where epigenetic alterations are prevalent. The generation of BRPF1 recombinant proteins also enables high-throughput screening of small molecules that can modulate its activity, paving the way for potential drug development. Overall, research on BRPF1 not only enhances our comprehension of chromatin biology but also holds promise for advancing therapeutic strategies in diseases linked to epigenetic dysregulation.












