Analytical Data
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Gene name
BRPF1
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简介
The BRPF1 protein is a key scaffold in HAT complexes such as MOZ/MORF and HBO1, and has histone H3 acetyltransferase activity. In the HBO1 complex, BRPF1 directs the specificity of KAT7/HBO1 for H3K14ac. BRPF1 Protein, Human is the recombinant human-derived BRPF1 protein, expressed by E. coli , with tag free.
- Application
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Alternative Names
BRPF1; Peregrin; Bromodomain and PHD finger-containing protein 1; Protein Br140
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Species
Human
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P55201
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Expression Region
E1079-S1207
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Protein Length
Partial
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
BRPF1 (Bromodomain and PHD Finger Containing 1) is a protein that plays a crucial role in chromatin remodeling and gene regulation, impacting various biological processes, including cell proliferation and differentiation. Emerging research has illuminated its involvement in multiple diseases, particularly cancers, where aberrations in its expression or function can lead to dysregulated cell growth. The study of BRPF1 is particularly relevant given its unique structural features, including bromodomains that recognize acetylated lysines on histones, which are vital for the epigenetic control of gene expression. Moreover, BRPF1 is a component of several protein complexes that facilitate transcriptional activation and chromatin modification, underscoring its significance in maintaining cellular homeostasis. Investigating BRPF1 as a recombinant protein allows researchers to explore its functional properties in detail, including binding affinities and interactions with other chromatin-associated proteins. Understanding the molecular mechanisms by which BRPF1 regulates gene expression could reveal novel therapeutic targets, especially in cancer where epigenetic alterations are prevalent. The generation of BRPF1 recombinant proteins also enables high-throughput screening of small molecules that can modulate its activity, paving the way for potential drug development. Overall, research on BRPF1 not only enhances our comprehension of chromatin biology but also holds promise for advancing therapeutic strategies in diseases linked to epigenetic dysregulation.
