Analytical Data
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Gene name
ACAT2
- Application
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Alternative Names
Acetyl-CoA transferase-like protein Cytosolic acetoacetyl-CoA thiolase
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Species
Human
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Source
E. coli
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Tag
N- His & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BWD1
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Expression Region
1-397aa
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Molecular Weight
48.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ACAT2, or Acyl-CoA:cholesterol acyltransferase 2, is an important enzyme involved in cholesterol esterification, playing a crucial role in lipid metabolism and homeostasis. It catalyzes the conversion of free cholesterol and fatty acyl-CoA into cholesterol esters, which are essential for cellular functions, including membrane structure, energy storage, and signaling. Dysregulation of ACAT2 has been implicated in various diseases, such as atherosclerosis, obesity, and neurodegenerative disorders. Given its significance in lipid metabolism, ACAT2 has emerged as a potential therapeutic target for the treatment of these conditions. The study of recombinant ACAT2 proteins allows for detailed investigations into the enzyme's structure, function, and regulatory mechanisms. Producing ACAT2 recombinantly enables researchers to explore its biochemical properties, assess its interactions with potential inhibitors, and develop assays for high-throughput screening of drug candidates. Furthermore, understanding ACAT2's role in cellular cholesterol homeostasis and its involvement in pathological processes can elucidate the underlying mechanisms of lipid-related diseases and facilitate the identification of novel therapeutic strategies. As research continues to uncover the complexities of lipid metabolism, ACAT2 remains a compelling focus within the field of metabolic disease research, with increasing relevance in the pursuit of effective treatments for associated health issues.
