Analytical Data
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Gene name
ANO1/Anoctamin-1
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简介
ANO1/Anoctamin-1 protein, a calcium-activated chloride channel (CaCC), is involved in ion transport, smooth muscle contraction, and mucus secretion. ANO1/Anoctamin-1 Protein, Human (N-His, C-Myc) is the recombinant human-derived ANO1/Anoctamin-1 protein, expressed by E. coli , with C-Myc, N-10*His labeled tag.
- Application
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Species
Human
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Source
E. coli
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Tag
C-Myc;N-10*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q5XXA6-1
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Expression Region
S806-A892
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Protein Length
Partial
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Molecular Weight
17.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ANO1, also known as Anoctamin-1 or TMEM16A, is a calcium-activated chloride channel that plays a crucial role in various physiological processes, including epithelial fluid secretion, smooth muscle contraction, and neuronal signaling. Its expression is particularly notable in tissues such as the salivary glands, intestines, and respiratory tract, where it contributes to the regulation of secretory functions and mucus production. The dysregulation of ANO1 has been linked to several pathological conditions, including asthma, cystic fibrosis, and cancer, making it a significant target for therapeutic interventions. Research on recombinant ANO1 protein has gained traction in recent years as scientists seek to better understand its structure, function, and regulatory mechanisms. Producing ANO1 in a recombinant form allows for detailed biochemical studies, including ion channel activity assays and drug screening, thereby providing insights into potential pharmacological applications. Furthermore, elucidating the molecular basis of ANO1’s activity could pave the way for novel treatments that target ANO1-related disorders. As such, the study of recombinant ANO1 not only contributes to fundamental biological knowledge but also holds promise for advancing medical science and therapeutic strategies in areas impacted by ANO1 dysfunction.
