Analytical Data
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基因名
USP8
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简介
USP8 is a key hydrolase in cellular regulation and plays a crucial role in protein turnover by efficiently deubiquitinating proteins and preventing degradation. Its activity spans "Lys-48" and "Lys-63" linked ubiquitin chains, highlighting its versatility. USP8 Protein, Human (sf9, His, GST) is the recombinant human-derived USP8 protein, expressed by Sf9 insect cells, with N-His and N-GST labeled tag.
- Application
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别名
Ubiquitin carboxyl-terminal hydrolase 8; Deubiquitinating enzyme 8; Ubiquitin isopeptidase Y (hUBPy); Ubiquitin thioesterase 8; Ubiquitin-specific-processing protease 8; KIAA0055; UBPY
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种属
Human
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表达系统
Baculovirus
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标签
N-His;N-GST
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P40818
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表达区间
P734-G1110
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氨基酸序列
PTVTPTVNRENKPTCYPKAEISRLSASQIRNLNPVFGGSGPALTGLRNLGNTCYMNSILQCLCNAPHLADYFNRNCYQDDINRSNLLGHKGEVAEEFGIIMKALWTGQYRYISPKDFKITIGKINDQFAGYSQQDSQELLLFLMDGLHEDLNKADNRKRYKEENNDHLDDFKAAEHAWQKHKQLNESIIVALFQGQFKSTVQCLTCHKKSRTFEAFMYLSLPLASTSKCTLQDCLRLFSKEEKLTDNNRFYCSHCRARRDSLKKIEIWKLPPVLLVHLKRFSYDGRWKQKLQTSVDFPLENLDLSQYVIGPKNNLKKYNLFSVSNHYGGLDGGHYTAYCKNAARQRWFKFDDHEVSDISVSSVKSSAAYILFYTSLG
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蛋白长度
Partial
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
USP8 (Ubiquitin-Specific Protease 8) is an important deubiquitinating enzyme that plays a pivotal role in the regulation of various cellular processes by removing ubiquitin moieties from target proteins, thus controlling their stability and activity. Dysregulation of USP8 has been linked to various diseases, including cancers and neurodegenerative disorders. As a result, understanding its structure and function is crucial for elucidating its biological roles and potential as a therapeutic target. Recent research has revealed that USP8 is involved in key signaling pathways, including those governing cell cycle progression and apoptosis, while mutations in the USP8 gene have been associated with Cushing's disease, a condition characterized by excessive cortisol production. The expression and activity of USP8 can be influenced by various factors, including post-translational modifications and protein-protein interactions, making it a complex but fascinating subject of study. The recombination and purification of USP8 protein for functional assays and structural studies are essential for advancing our knowledge of its mechanisms. Investigating the enzymatic activity of USP8 and its impact on substrate proteins can provide insights into how its dysregulation contributes to disease pathogenesis and may guide the development of novel therapeutic strategies. In light of its significance, ongoing research aims to elucidate the molecular mechanisms underlying USP8's functions, offering the potential for innovative approaches to modulate its activity in disease contexts.
