Analytical Data
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Gene name
Histone H3
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简介
Histone H3 proteins are critical in nucleosomes, which compact DNA into chromatin and regulate DNA accessibility. Histone H3 Protein, Human (His) is the recombinant human-derived Histone H3 protein, expressed by E. coli , with N-His labeled tag.
- Application
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Alternative Names
Histone H3.1; Histone H3
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P68431
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Expression Region
A2-A136
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Protein Length
Full Length of Mature Protein
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Molecular Weight
18 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Histone H3 recombinant protein has garnered significant interest in the field of molecular biology due to its critical role in chromatin structure and gene regulation. Histones, particularly H3, are fundamental components of nucleosomes, which package DNA into a compact, organized form within the nucleus of eukaryotic cells. The post-translational modifications of histone H3, such as methylation, acetylation, and phosphorylation, are known to influence gene expression patterns and are closely linked to various cellular processes, including replication, repair, and transcription. The study of recombinant Histone H3 allows researchers to explore these modifications in a controlled environment, facilitating their understanding of epigenetic mechanisms. Furthermore, aberrations in histone modifications have been associated with several diseases, including cancer, making the investigation of H3 variants and their interactions crucial for therapeutic development. By utilizing recombinant technology, scientists can produce specific histone variants for in vitro studies, enabling them to dissect the influence of particular histone modifications on chromatin dynamics and gene activity. Ultimately, this research contributes to a deeper understanding of the epigenetic landscape and its implications in health and disease.
