Analytical Data
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Gene name
cGAS
- Application
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Alternative Names
C6orf150; cGAS; Cyclic GMP-AMP synthase
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q8N884
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Expression Region
Glu225~Cys405
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Molecular Weight
24kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Cyclic GMP-AMP synthase (cGAS) is a crucial cytosolic DNA sensor that plays a pivotal role in the innate immune response. The discovery of cGAS was driven by the need to understand how the immune system detects foreign DNA, particularly from viruses and bacteria. Upon recognizing double-stranded DNA (dsDNA) in the cytosol, cGAS catalyzes the synthesis of cyclic GMP-AMP (cGAMP), a second messenger that activates the STING (Stimulator of Interferon Genes) pathway, leading to the production of type I interferons and pro-inflammatory cytokines. This pathway is essential for mounting an effective immune response against infections and for influencing the fate of tumor cells. The study of recombinant cGAS protein has become increasingly important in both basic and applied research, as it allows researchers to investigate its structure-function relationships, enzymatic activity, and regulatory mechanisms. Moreover, cGAS’s role in various diseases, including autoimmune disorders, cancer, and viral infections, highlights its potential as a therapeutic target. Understanding the biochemical properties of recombinant cGAS can facilitate the development of novel immunotherapeutic strategies and diagnostic tools, underscoring the significance of this protein in immunology and molecular biology.
