Analytical Data
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基因名
pulB
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简介
PulB is a protein belonging to the glycosyl hydrolase 13 family. The glycosyl hydrolase 13 family contains enzymes involved in the breakdown of complex carbohydrates by catalyzing the hydrolysis of glycosidic bonds. pulB Protein, Pullulanibacillus naganoensis is the recombinant pulB protein, expressed by E. coli , with tag free.
- Application
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别名
pulB; Alpha-dextrin endo-1; 6-alpha-glucosidase; Pullulan 6-glucanohydrolase
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种属
Others
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表达系统
E. coli
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标签
Tag Free
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
G9JLV4
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表达区间
D1-K926
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蛋白长度
Full Length
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PulB is a key protein involved in the Type II secretion system (T2SS) utilized by various Gram-negative bacteria to transport proteins across the outer membrane. This system plays a crucial role in the pathogenicity of several species, including Pseudomonas aeruginosa, by enabling the secretion of virulence factors and extracellular enzymes that enhance survival and infection. The PulB protein specifically functions as a component of the Pul system, which is essential for the secretion of specific proteins involved in biofilm formation and nutrient acquisition. Research on PulB has garnered significant attention due to its implications in microbial physiology and its potential as a target for antibacterial strategies. Understanding the structure and function of PulB can provide insights into the mechanistic details of the T2SS, revealing how bacteria adapt to environmental challenges and contribute to their pathogenic traits. Characterizing PulB recombinant proteins allows scientists to explore its interaction with other secretion components and to assess its role in the protein export process. This research is not only vital for understanding bacterial biology but also holds promise for developing novel therapeutic approaches aimed at disrupting the secretion mechanisms that contribute to bacterial virulence. As antibiotic resistance becomes a growing concern, targeting the secretion systems may represent a promising avenue for innovative antimicrobial therapies. Thus, the study of PulB and its associated pathways is essential for advancing our understanding of bacterial pathogenesis and designing effective interventions against infectious diseases.
