Analytical Data
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Gene name
USP44
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简介
The USP44 protein is a key deubiquitinase that regulates the spindle assembly checkpoint and prevents premature initiation of anaphase by deubiquitinating CDC20. This stabilizes the MAD2L1-CDC20-APC/C complex and enhances the spindle assembly checkpoint. USP44 Protein, Human is the recombinant human-derived USP44 protein, expressed by E. coli , with tag free.
- Application
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Alternative Names
USP44; Ubiquitin carboxyl-terminal hydrolase 44; Deubiquitinating enzyme 44; Ubiquitin thioesterase 44; Ubiquitin-specific-processing protease 44
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Species
Human
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9H0E7
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Expression Region
M1-S712
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Protein Length
Full Length
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
USP44 (Ubiquitin Specific Peptidase 44) is an important member of the ubiquitin-specific protease family, known for its role in regulating cellular processes through the removal of ubiquitin moieties from target proteins. Research into USP44 has garnered significant attention due to its critical involvement in cell cycle regulation, particularly in the maintenance of genomic stability. Recent studies have highlighted its function in preventing premature anaphase onset by deubiquitinating key substrates involved in the anaphase-promoting complex/cyclosome (APC/C) pathway, thereby influencing the degradation of proteins necessary for chromosome segregation. Additionally, aberrant expression or mutation of USP44 has been implicated in various cancers, suggesting its potential role as a tumor suppressor and a prospective target for therapeutic interventions. The exploration of USP44's structure and function, including its enzymatic mechanisms, has opened avenues for developing specific inhibitors that can modulate its activity. Moreover, understanding the interplay between USP44 and other cellular signaling pathways is essential for elucidating its comprehensive role in cell biology and pathology. This underscores the significance of USP44 in both basic research and potential clinical applications, making it a valuable focus for ongoing studies in cancer biology and therapeutic research.
