Analytical Data
-
基因名
EXO I
-
简介
EXO I Protein, documented with high processivity, degrades single-stranded DNA (ssDNA). It also acts as a DNA deoxyribophosphodiesterase, releasing deoxyribose-phosphate moieties post-cleavage at apurinic/apyrimidinic (AP) sites by AP endonuclease or AP lyase. EXO I's dual functionality in ssDNA degradation and deoxyribose-phosphate removal at AP sites underscores its crucial role in DNA repair processes, maintaining genome integrity. EXO I Protein, E. coli (His) is the recombinant E. coli-derived EXO I protein, expressed by E. coli , with N-6*His labeled tag. EXO I Protein, E. coli (His), has molecular weight of ~54.5 kDa.
- Application
-
种属
E.coli
-
表达系统
E. coli
-
标签
N-6*His
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
氨基酸序列
MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNIFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGLPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLVQQYADDKEKVALLKALWQYAEEIV
-
分子量
54.5 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
EXO I, or Exonuclease I, is a crucial enzyme that plays a significant role in various molecular biology applications, particularly in DNA manipulation and analysis. Traditionally, EXO I is known for its ability to specifically degrade single-stranded DNA in a 3' to 5' direction, making it an invaluable tool in procedures such as cloning, sequencing, and other genomic studies. The increasing interest in precision medicine and genetic engineering has driven the need for improved understanding and optimization of EXO I functions. Researchers have focused on characterizing its enzymatic properties, understanding its interaction with different DNA structures, and exploring its applications to enhance the efficiency of PCR (Polymerase Chain Reaction) and other nucleic acid amplification techniques. Furthermore, the potential to engineer and optimize EXO I's activity opens up exciting possibilities for innovative applications in diagnostics and therapeutics, particularly in the context of targeted treatments for genetic disorders and cancer. Overall, the research surrounding EXO I not only contributes to the fundamental understanding of molecular biology but also paves the way for advancements in biotechnology and medicine.
