Analytical Data
-
Gene name
ABHD4
-
简介
ABHD4 is a lysophospholipase that acts selectively on N-acylphosphatidylethanolamines (NAPE), which is essential for the biosynthesis of N-acylphosphatidylethanolamines such as anandamide. It hydrolyzes the sn-1 and sn-2 acyl chains in NAPE to generate glycerophosphate-N-acylethanolamine (GP-NAE), which is an important intermediate in the production of N-acylethanolamine. ABHD4 Protein, Human (sf9, His) is the recombinant human-derived ABHD4 protein, expressed by Sf9 insect cells , with N-His labeled tag.
- Application
-
Alternative Names
(Lyso)-N-acylphosphatidylethanolamine lipase; Alpha/beta-hydrolase 4; ABHD4
-
Species
Human
-
Source
Baculovirus
-
Tag
N-His
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q8TB40
-
Expression Region
M1-D342
-
Protein Length
Full Length
-
Molecular Weight
40 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ABHD4 (abhydrolase domain-containing protein 4) is an intriguing member of the serine hydrolase family, involved in lipid metabolism and signaling pathways. Research on ABHD4 is rooted in its potential role in the regulation of various physiological processes, including inflammation and neurological functions. The enzyme is believed to participate in the hydrolysis of endocannabinoids, which are endogenous lipids that bind to cannabinoid receptors and influence several cellular functions. Dysregulation of ABHD4 has been associated with numerous health conditions, including neurodegenerative diseases and metabolic disorders, making it a potential therapeutic target. Recent studies have focused on the structural characterization and functional analysis of recombinant ABHD4 proteins to elucidate their substrate specificity and catalytic mechanisms. Understanding these aspects could pave the way for designing specific inhibitors or modulators, offering novel strategies for drug development. Given its diverse biological roles and implications in health, the study of ABHD4 recombinant proteins is essential for uncovering new insights into lipid signaling and its relevance to human diseases.
