Analytical Data
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Gene name
NEK9
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简介
The NEK9 protein is a pleiotropic mitotic regulator that oversees spindle dynamics and chromosome segregation. It phosphorylates a variety of substrates, including histones, myelin basic protein, β-casein, and BICD2. NEK9 Protein, Human (sf9, GST) is the recombinant human-derived NEK9 protein, expressed by sf9 insect cells , with N-GST labeled tag.
- Application
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Alternative Names
NEK9; Serine/threonine-protein kinase Nek9; Nercc1 kinase; Never in mitosis A-related kinase 9; NimA-related protein kinase 9; NimA-related kinase 8; Nek8
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Species
Human
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Source
Baculovirus
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Tag
N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8TD19 (S2-L979);
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Expression Region
ΔT346-K731
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Protein Length
Full Length of Mature Protein
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Molecular Weight
91.6 KDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NEK9, a member of the Never in Mitosis Gene A (NIMA)-related kinase family, plays a crucial role in cell cycle regulation, particularly during mitosis. It has garnered attention due to its involvement in various cellular processes, including centrosome function, spindle assembly, and cytokinesis. Dysregulation of NEK9 is implicated in several cancers, making it a potential target for therapeutic interventions. Research has shown that NEK9 interacts with various proteins that are essential for mitotic progression, highlighting its significance in maintaining genomic integrity. Furthermore, NEK9's kinase activity and its regulation through phosphorylation are critical for its function, prompting investigations into the development of small-molecule inhibitors. As a recombinant protein, NEK9 provides a valuable tool for studying its biological activities and interactions in vitro, enabling researchers to elucidate its precise role in oncogenic pathways. Understanding NEK9's function and regulation could lead to innovative strategies for cancer treatment, making it a focal point in cell biology and cancer research.
