Analytical Data
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基因名
NEK9
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简介
The NEK9 protein is a pleiotropic mitotic regulator that oversees spindle dynamics and chromosome segregation. It phosphorylates a variety of substrates, including histones, myelin basic protein, β-casein, and BICD2. NEK9 Protein, Human (sf9, GST) is the recombinant human-derived NEK9 protein, expressed by sf9 insect cells , with N-GST labeled tag.
- Application
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别名
NEK9; Serine/threonine-protein kinase Nek9; Nercc1 kinase; Never in mitosis A-related kinase 9; NimA-related protein kinase 9; NimA-related kinase 8; Nek8
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种属
Human
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表达系统
Baculovirus
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标签
N-GST
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8TD19 (S2-L979);
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表达区间
ΔT346-K731
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蛋白长度
Full Length of Mature Protein
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分子量
91.6 KDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NEK9, a member of the Never in Mitosis Gene A (NIMA)-related kinase family, plays a crucial role in cell cycle regulation, particularly during mitosis. It has garnered attention due to its involvement in various cellular processes, including centrosome function, spindle assembly, and cytokinesis. Dysregulation of NEK9 is implicated in several cancers, making it a potential target for therapeutic interventions. Research has shown that NEK9 interacts with various proteins that are essential for mitotic progression, highlighting its significance in maintaining genomic integrity. Furthermore, NEK9's kinase activity and its regulation through phosphorylation are critical for its function, prompting investigations into the development of small-molecule inhibitors. As a recombinant protein, NEK9 provides a valuable tool for studying its biological activities and interactions in vitro, enabling researchers to elucidate its precise role in oncogenic pathways. Understanding NEK9's function and regulation could lead to innovative strategies for cancer treatment, making it a focal point in cell biology and cancer research.
