Analytical Data
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Gene name
MELK
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简介
MELK is a multifunctional serine/threonine protein kinase that regulates the cell cycle, stem cell self-renewal, apoptosis, and splicing. MELK phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622, activates apoptosis through MAP3K5/ASK1, helps CDC25B localize during mitosis, and inhibits BCL2L14, which may affect breast cancer occurrence. MELK Protein, Human (His) is the recombinant human-derived MELK protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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Alternative Names
MELK; Maternal embryonic leucine zipper kinase; hMELK; Protein kinase Eg3; pEg3 kinase; Protein kinase PK38; hPK38; Tyrosine-protein kinase MELK
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q14680
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Expression Region
D3-V330
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Protein Length
Partial
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Molecular Weight
39.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MELK (maternal embryonic leucine zipper kinase) is a serine/threonine protein kinase that has garnered significant attention in recent years due to its intriguing role in various cellular processes, particularly in cancer biology. Initially identified as a maternal protein crucial for early embryonic development, MELK's expression is noted to be upregulated in numerous cancer types, suggesting its involvement in tumorigenesis and progression. Research has shown that MELK can influence cell proliferation, survival, and differentiation, making it a promising target for cancer therapy. Additionally, studies have revealed that MELK interacts with various signaling pathways and proteins, further highlighting its importance in cellular regulation. The distinct expression patterns of MELK in tumors compared to normal tissues provide a potential biomarker for cancer diagnosis and prognosis. As a result, extensive efforts are underway to understand the molecular mechanisms underlying MELK's function and to explore its potential as a therapeutic target, particularly for cancers that exhibit resistance to conventional treatments. Overall, MELK's dual role in developmental biology and cancer has positioned it as a focus of intense research, with the aim of uncovering novel strategies for cancer intervention and improving patient outcomes.
