Analytical Data
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Gene name
IKKε
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简介
IKKε protein is a serine/threonine kinase that coordinates the inflammatory response to viral infection by activating type I IFN, NF-kappa-B, and STAT signaling pathways. Upon activation of the viral RNA sensor, IKKε binds to DDX3X and phosphorylates IRF3, IRF7, and DDX3X, resulting in IRF3 nuclear translocation. IKKε Protein, Human (sf9, GST) is the recombinant human-derived IKKε protein, expressed by sf9 insect cells , with N-GST labeled tag.
- Application
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Alternative Names
IKKε; Inhibitor of nuclear factor kappa-B kinase subunit epsilon; I-kappa-B kinase epsilon; IKK-E; IKK-epsilon; IkBKE; Inducible I kappa-B kinase; IKK-i
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Species
Human
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Source
Baculovirus
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Tag
N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q14164-1
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Expression Region
Q2-V716
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Protein Length
Partial
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Molecular Weight
106.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
IKKε (Inhibitor of kappa B kinase epsilon) is a critical component of the NF-kB signaling pathway, which plays a significant role in regulating immune responses, inflammation, and cell survival. It has garnered considerable attention in recent years due to its involvement in various pathophysiological conditions, including cancer, autoimmune diseases, and viral infections. The activation of IKKε leads to the phosphorylation and degradation of IκB proteins, thereby releasing NF-kB dimers that translocate to the nucleus and initiate the transcription of target genes. The study of recombinant IKKε proteins has become essential for understanding the precise mechanisms of NF-kB activation and its downstream effects. Recombinant proteins allow researchers to dissect the functional domains of IKKε, explore its interaction with other signaling molecules, and investigate its potential as a therapeutic target. Furthermore, advances in recombinant DNA technology have facilitated the production of IKKε proteins in various expression systems, aiding in the functional assays and the development of IKKε inhibitors. As our understanding of IKKε continues to expand, it holds promise for novel strategies in treating diseases associated with aberrant NF-kB signaling, making it a focal point of ongoing research in molecular biology and pharmacology.
