Analytical Data
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Gene name
PK3C3
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简介
The PI3KC3 protein is the catalytic subunit of the PI3K complex and mediates the formation of phosphatidylinositol 3-phosphate. PI3KC3-C1 initiates autophagosomes, whereas PI3KC3-C2 promotes autophagosome maturation and endocytosis. PK3C3 Protein, Human (sf9, His, GST) is the recombinant human-derived PK3C3 protein, expressed by sf9 insect cells , with N-8*His, N-GST labeled tag.
- Application
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Alternative Names
PIK3C3; Phosphatidylinositol 3-kinase catalytic subunit type 3; PI3-kinase type 3; PI3K type 3; PtdIns-3-kinase type 3; Phosphatidylinositol 3-kinase p100 subunit; Phosphoinositide-3-kinase class 3; hVps34
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Species
Human
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Source
Baculovirus
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Tag
N-8*His;N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8NEB9
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Expression Region
G2-K887
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Protein Length
Partial
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Molecular Weight
129.2 KDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PK3C3 is a recombinant protein that has garnered significant attention in the field of molecular biology and biochemistry due to its potential roles in cellular processes such as autophagy and signal transduction. The protein derives its name from the PKC isoforms, specifically protein kinase C, which are crucial in various cellular signaling pathways related to growth, differentiation, and apoptosis. Research indicates that PK3C3 plays a pivotal role in the regulation of autophagic processes, linking energy metabolism and cell survival under stress conditions. Studies have shown that PK3C3 interacts with various cellular machinery and proteins, influencing the overall cellular homeostasis and response to environmental stimuli. Furthermore, its recombinant version allows for thorough functional studies and potential therapeutic exploration, given its involvement in diseases such as cancer and neurodegenerative disorders. The ability to produce PK3C3 in the laboratory has opened new avenues for exploring its mechanistic roles and examining its potential as a biomarker or therapeutic target. Its study contributes not only to the understanding of fundamental biological processes but also to the development of novel strategies in disease treatment and prevention. This makes PK3C3 a significant protein in ongoing research endeavors aimed at unraveling the complexities of cellular functions and their implications in health and disease.
