Analytical Data
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Gene name
Cas9
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简介
The CRISPR-Cas9 protein is part of an immune system that defends against genetic elements. It processes RNA and aids in cleaving DNA targets. Protein and guide RNAs are necessary for its function. The protein recognizes specific sequences to distinguish self from nonself and provides immunity against matching genetic elements. Cas9 Protein, Streptococcus pyogenes serotype M1 is the recombinant Cas9, expressed by E. coli , with tag Free labeled tag.
- Application
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Alternative Names
csn1
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Species
Others
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q99ZW2
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Expression Region
D2-D1368
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Protein Length
Partial
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Molecular Weight
160 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Cas9 recombinant protein has emerged as a pivotal tool in the field of molecular biology, particularly in the realm of genome editing. Originally derived from the adaptive immune system of Streptococcus pyogenes, Cas9 functions as an RNA-guided endonuclease, capable of introducing site-specific double-strand breaks in DNA. This unique ability has propelled the development of the CRISPR-Cas9 technology, revolutionizing genetic engineering and therapeutic applications. Researchers have been intensively investigating the structural and functional aspects of Cas9 to enhance its specificity and efficiency, as well as to expand its utility across diverse organisms. The study of Cas9 also involves optimizing delivery methods, developing engineered variants, and understanding its interactions with guide RNA and target DNA. These advances promise to facilitate precise gene editing strategies for treating genetic disorders, improving agricultural traits, and investigating gene function. By elucidating the mechanisms underlying Cas9 activity, researchers aim to refine its application and address potential off-target effects, fostering a deeper understanding of genome dynamics and paving the way for innovative biotechnological solutions.
