Analytical Data
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Gene name
ERK1
- Application
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Alternative Names
ERT2Extracellular domain signal-regulated kinase 1 ;ERK-1Insulin-stimulated MAP2 kinase;MAP kinase isoform p44 ;p44-MAPKMicrotubule-associated protein 2 kinasep44-ERK1
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Species
Human
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Source
E. coli
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Tag
N- His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P27361
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Expression Region
11-379aa
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Molecular Weight
46.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ERK1 (Extracellular signal-Regulated Kinase 1) is a critical component of the mitogen-activated protein kinase (MAPK) signaling pathway, playing a vital role in various cellular processes such as proliferation, differentiation, and survival. Research has shown that ERK1 is activated in response to growth factors and stress stimuli, leading to the phosphorylation of downstream substrates that ultimately regulate gene expression. Understanding the function and regulation of ERK1 is essential for elucidating its role in cancer biology, as aberrant ERK signaling is often implicated in tumorigenesis and disease progression. The production of recombinant ERK1 proteins has been crucial for studying its biochemical properties, post-translational modifications, and interactions with other cellular molecules. Moreover, by generating ERK1 in a controlled environment, researchers can perform detailed assays to uncover the nuances of its activity and regulation, paving the way for potential therapeutic interventions in diseases characterized by dysregulated MAPK signaling. Thus, the study of recombinant ERK1 proteins not only enhances our comprehension of fundamental cellular mechanisms but also potentially provides insights for drug development and targeted therapies in oncology.
