Analytical Data
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Gene name
SIRT3
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简介
SIRT3 is an NAD-dependent protein deacetylase that dynamically affects the acetylation of mitochondrial target proteins, such as ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA, and the ATP synthase subunit ATP5PO. Its deacetylation activity regulates cellular energy metabolism and fine-tunes tissue-specific ATP levels. SIRT3 Protein, Human (His, SUMO) is the recombinant human-derived SIRT3 protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag.
- Application
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Alternative Names
SIRT3; NAD-dependent protein deacetylase sirtuin-3; mitochondrial; hSIRT3; Regulatory protein SIR2 homolog 3; SIR2-like protein 3
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Species
Human
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Source
E. coli
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Tag
N-6*His;N-SUMO
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NTG7
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Expression Region
S118-K399
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Molecular Weight
44.4 KDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SIRT3, a member of the sirtuin family of proteins, has garnered significant attention in recent years due to its critical role in regulating metabolic processes and cellular stress responses. As a mitochondrial deacetylase, SIRT3 is involved in the desacetylation of key metabolic enzymes, thereby influencing energy expenditure, oxidative stress, and overall mitochondrial function. Research has illuminated its implications in age-related diseases, including obesity, diabetes, and neurodegenerative disorders, making it a potential target for therapeutic interventions. Studies have demonstrated that SIRT3 activation can enhance mitochondrial function, improve insulin sensitivity, and reduce inflammation, which are crucial in maintaining cellular homeostasis. Furthermore, the exploration of SIRT3 recombinant proteins has provided a valuable tool for understanding its enzymatic mechanisms and identifying potential substrates. These insights could lead to the development of SIRT3-modulating drugs, with the aim of improving health span and combating age-related pathologies. Overall, SIRT3's role in metabolism and cellular health places it at the forefront of current biomedical research, reflecting the importance of mitochondrial regulation in disease prevention and treatment.
