Analytical Data
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Gene name
NEDD4L
- Application
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Alternative Names
(HECT-type E3 ubiquitin transferase NED4L)(NEDD4.2)(Nedd4-2)
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Species
Human
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Source
E. coli
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Tag
N- GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96PU5
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Expression Region
2-975aa
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Molecular Weight
138.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NEDD4L, a member of the NEDD4 family of E3 ubiquitin ligases, has garnered considerable attention in recent years due to its crucial role in regulating various cellular processes, including protein degradation, trafficking, and signaling pathways. It is involved in the ubiquitin-proteasome system, which is essential for maintaining cellular homeostasis by controlling the degradation of proteins tagged for destruction. Dysregulation of NEDD4L has been implicated in several human diseases, including hypertension, cancer, and metabolic disorders, highlighting its potential as a therapeutic target. Research into recombinant NEDD4L proteins aims to better understand its functional mechanisms and interactions at the molecular level. By producing and characterizing recombinant NEDD4L, scientists can elucidate its structure-function relationships, identify binding partners, and explore its enzymatic activity. Ultimately, this knowledge may facilitate the development of NEDD4L-targeted drugs and provide insights into the broader implications of ubiquitination in health and disease. Furthermore, recombinant proteins serve as valuable tools for high-throughput screening and in vivo studies, thereby enhancing our understanding of NEDD4L's role in various biological contexts.
