Analytical Data
-
基因名
GFPT1
-
简介
As a key regulator of glucose influx into the hexosamine pathway, the GFPT1 protein plays a key role in controlling the availability of protein N- and O-linked glycosylation precursors. In addition, GFPT1 is involved in the regulation of circadian expression of clock genes BMAL1 and CRY1 and contributes to the complex regulation of cytoplasmic UDP-GlcNAc metabolic fluctuations. GFPT1 Protein, Human is the recombinant human-derived GFPT1 protein, expressed by E. coli , with tag free.
- Application
-
别名
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1; GFAT1; GFAT; GFPT
-
种属
Human
-
表达系统
E. coli
-
标签
Tag Free
-
纯度
Greater than 95% as determined by SDS-PAGE.
-
蛋白编号
Q06210-1
-
表达区间
Q332-E699
-
氨基酸序列
QQIMKGNFSSFMQKEIFEQPESVVNTMRGRVNFDDYTVNLGGLKDHIKEIQRCRRLILIACGTSYHAGVATRQVLEELTELPVMVELASDFLDRNTPVFRDDVCFFLSQSGETADTLMGLRYCKERGALTVGITNTVGSSISRETDCGVHINAGPEIGVASTKAYTSQFVSLVMFALMMCDDRISMQERRKEIMLGLKRLPDLIKEVLSMDDEIQKLATELYHQKSVLIMGRGYHYATCLEGALKIKEITYMHSEGILAGELKHGPLALVDKLMPVIMIIMRDHTYAKCQNALQQVVARQGRPVVICDKEDTETIKNTKRTIKVPHSVDCLQGILSVIPLQLLAFHLAVLRGYDVDFPRNLAKSVTVE
-
蛋白长度
Partial
-
分子量
41.5 kDa.
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GFPT1 (Glutamine-Fructose-6-Phosphate Transaminase 1) is an important enzyme in the hexosamine biosynthetic pathway, which plays a crucial role in cellular metabolism and signaling. It catalyzes the conversion of fructose-6-phosphate and glutamine to glucosamine-6-phosphate, a precursor for various glycosylation processes. Aberrant GFPT1 activity has been linked to several metabolic disorders, including obesity, diabetes, and cancer. Research has highlighted its potential as a biomarker for these conditions, as well as its involvement in the modulation of insulin signaling and inflammation. In addition, GFPT1 has garnered attention for its role in the development of complications related to diabetes, such as neuropathy and retinopathy, by influencing the production of advanced glycation end-products (AGEs). Understanding the molecular mechanisms underlying GFPT1's function and regulation is essential for the development of targeted therapies aimed at metabolic diseases. Moreover, the production of recombinant GFPT1 protein has become a focal point for studying its biochemical properties and interactions within the cell, providing insights into its therapeutic potential and paving the way for novel interventions in metabolic dysfunction.
