Analytical Data
-
基因名
CHST3
-
简介
The CHST3 protein utilizes PAPS to keyly catalyze the transfer of sulfate to position 6 of GalNAc in chondroitin, the major proteoglycan in cartilage. CHST3 also sulfonates Gal residues in keratan sulfate and sialic acid LacNAc oligosaccharides with less efficiency. CHST3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CHST3 protein, expressed by HEK293 , with N-His labeled tag.
- Application
-
别名
Carbohydrate sulfotransferase 3; Chondroitin 6-O-sulfotransferase 1; C6ST-1; GST-0
-
种属
Mouse
-
表达系统
HEK293
-
标签
N-10*His
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
O88199
-
表达区间
E39-T472
-
氨基酸序列
EKENKIISRVSDKLKQIPHFVADANSTDPALLLSENASLLSLSELDSTFSHLRSRLHNLSLQLGVEPAMESQEAGAEKPSQQAGAGTRRHVLLMATTRTGSSFVGEFFNQQGNIFYLFEPLWHIERTVFFQQRGASAAGSALVYRDVLKQLLLCDLYVLEPFISPPPEDHLTQFLFRRGSSRSLCEDPVCTPFVKKVFEKYHCRNRRCGPLNVTLAGEACRRKDHVALKAVRIRQLEFLQPLVEDPRLDLRVIQLVRDPRAVLASRIVAFAGKYENWKKWLSEGQDQLSEDEVQRLRGNCESIRLSAELGLRQPAWLRGRYMLVRYEDVARRPLQKAREMYSFAGIPLTPQVEDWIQKNTQATRDSSDVYSTQKNSSEQFEKWRFSMPFKLAQVVQAACGPTMHLFGYKLARDAASLTNRSISLLEERGTFWVT
-
蛋白长度
Lumenal Domain
-
分子量
75-80 kDa.
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CHST3 (Carbohydrate Sulfotransferase 3) is a critical enzyme involved in the sulfation of carbohydrate moieties, particularly in glycosaminoglycans (GAGs) such as heparan sulfate and chondroitin sulfate. Sulfation is a vital post-translational modification that affects various biological processes, including cell signaling, growth factor activity, and cellular interactions. Research into CHST3 has gained attention due to its potential role in developmental biology and disease mechanisms, particularly in cancer and congenital disorders where sulfation patterns are disrupted. Understanding CHST3's function and regulation can provide insights into its contribution to the extracellular matrix and the overall cellular microenvironment. Moreover, recombinant CHST3 protein is crucial for elucidating its biochemical properties, substrate specificity, and interaction with other proteins. Advances in protein engineering and expression systems have enabled the production of active CHST3, facilitating functional assays to explore its role in sulfation processes. This research holds promise not only for basic biological understanding but also for the development of therapeutic strategies targeting sulfation-related pathways in various diseases.
