Analytical Data
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基因名
CHST15
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简介
The CHST15 protein is a sulfotransferase that catalyzes the transfer of sulfate to GalNAc 4-sulfate in chondroitin sulfate A to form chondroitin sulfate E with GlcA-GalNAc(4,6-SO(4)) units. It also sulfates the unique non-reducing terminal sequence GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), similar to thrombomodulin chondroitin sulfate. CHST15 Protein, Human (HEK293, His) is the recombinant human-derived CHST15 protein, expressed by HEK293 , with N-His labeled tag.
- Application
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别名
Carbohydrate sulfotransferase 15; hBRAG; GalNAc4S-6ST; BRAG
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种属
Human
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表达系统
HEK293
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标签
N-10*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q7LFX5-1
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表达区间
S99-T561
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氨基酸序列
SGAHQELLISSPFHYGGFPSNPSLMDSENPSDTKEHHHQSSVNNISYMKDYPSIKLIINSITTRIEFTTRQLPDLEDLKKQELHMFSVIPNKFLPNSKSPCWYEEFSGQNTTDPYLTNSYVLYSKRFRSTFDALRKAFWGHLAHAHGKHFRLRCLPHFYIIGQPKCGTTDLYDRLRLHPEVKFSAIKEPHWWTRKRFGIVRLRDGLRDRYPVEDYLDLFDLAAHQIHQGLQASSAKEQSKMNTIIIGEASASTMWDNNAWTFFYDNSTDGEPPFLTQDFIHAFQPNARLIVMLRDPVERLYSDYLYFASSNKSADDFHEKVTEALQLFENCMLDYSLRACVYNNTLNNAMPVRLQVGLYAVYLLDWLSVFDKQQFLILRLEDHASNVKYTMHKVFQFLNLGPLSEKQEALMTKSPASNARRPEDRNLGPMWPITQKILRDFYRPFNARLAQVLADEAFAWKTT
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蛋白长度
Partial
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分子量
70-90 kDa.
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CHST15, or Carbohydrate Sulfotransferase 15, is an essential enzyme involved in the sulfation of carbohydrate moieties, particularly in the biosynthesis of glycosaminoglycans (GAGs) such as heparan sulfate and chondroitin sulfate. These GAGs play crucial roles in various biological processes, including cell signaling, development, and tissue repair. Abnormalities in the sulfation patterns of GAGs due to CHST15 deficiencies have been linked to several pathophysiological conditions, including cardiovascular diseases, cancer, and developmental disorders. As a result, understanding the structure and function of recombinant CHST15 protein is vital for elucidating its role in these processes. Moreover, the production of recombinant CHST15 offers a powerful tool for biochemical studies, enabling researchers to investigate its activity, substrate specificity, and interactions with other proteins. This research can lead to advances in therapeutic strategies targeting GAG-related diseases, as well as the development of novel biomaterials for regenerative medicine. Additionally, understanding the regulation of CHST15 and its impact on GAG sulfation may provide insights into potential biomarkers for disease diagnosis and prognosis. Thus, the study of recombinant CHST15 not only enhances our knowledge of fundamental biological mechanisms but also has significant implications for translational research in medicine.
