Analytical Data
-
基因名
PTEN
- Application
-
别名
BZS; MHAM; MMAC1; PTEN1; TEP1; Mutated In Multiple Advanced Cancers 1; Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
-
种属
Human
-
表达系统
E. coli
-
标签
N-terminal His-Tag
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
P60484
-
表达区间
2~403aa
-
氨基酸序列
TAIIKEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVR FLDSKHKNHYKIYNLCAERHYDTAKFNCRVAQYPFEDHNPPQLELIKPFCEDLD QWLSEDDNHVAAIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFYGEVRTRDK KGVTIPSQRRYVYYYSYLLKNHLDYRPVALLFHKMMFETIPMFSGGTCNPQFVV CQLKVKIYSSNSGPTRREDKFMYFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMF HFWVNTFFIPGPEETSEKVENGSLCDQEIDSICSIERADNDKEYLVLTLTKNDL DKANKDKANRYFSPNFKVKLYFTKTVEEPSNPEASSSTSVTPDVSDNEPDHYRY SDTTDSDPENEPFDEDQHTQITKV
-
分子量
48.6kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Identification
Protein Description
PTEN (Phosphatase and Tensin Homolog) is a critical tumor suppressor protein that plays a pivotal role in regulating cellular processes, including cell growth, survival, and proliferation, primarily through its lipid phosphatase activity that dephosphorylates phosphatidylinositol (3,4,5)-trisphosphate (PIP3). Mutations or deletions of the PTEN gene are commonly associated with various cancers, leading to the hyperactivation of the PI3K/Akt signaling pathway, which contributes to tumorigenesis. Due to its significant role in cancer biology, the study of PTEN has garnered considerable interest, particularly in understanding its functional mechanisms and potential as a therapeutic target. Researchers have focused on recombinant protein techniques to produce and characterize the PTEN protein, enabling the exploration of its structure-function relationships, subcellular localization, and interaction with other signaling molecules. This research aims to elucidate the dynamic role of PTEN in cellular signaling pathways and its implications in cancer development and progression. Furthermore, the development of PTEN-based therapies could pave the way for novel treatment strategies, particularly in tumors exhibiting PTEN deficiency. Current investigations are also examining the impact of PTEN reconstitution in cancer models, aiming to reverse the oncogenic effects caused by the loss of PTEN activity and enhance the efficacy of existing cancer therapeutics. Overall, the ongoing study of recombinant PTEN is crucial for uncovering its therapeutic potential and advancing our understanding of cancer biology.
