Analytical Data
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Gene name
LGP2
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简介
LGP2 protein is a key regulator of RIGI- and IFIH1/MDA5-mediated antiviral signaling and has dual negative and positive functions affected by factors such as infected viruses and target cells. LGP2 lacks a CARD domain for independent initiation of antiviral signaling and therefore inhibits RIG-I through multiple mechanisms, including competition for RNA binding and obstruction of interaction with MAVS/IPS1. LGP2 Protein, Human (HEK293, His, Strep, FLAG) is the recombinant human-derived LGP2 protein, expressed by HEK293 , with N-Strep, C-Flag, N-8*His labeled tag.
- Application
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Alternative Names
DHX58; Probable ATP-dependent RNA helicase DHX58; ATP-dependent helicase LGP2; Protein D11Lgp2 homolog; RIG-I-like receptor 3; RLR-3; RIG-I-like receptor LGP2; RLR
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Species
Human
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Source
HEK293
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Tag
N-StrepⅡ;N-8*His;C-Flag
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96C10
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Expression Region
M1-D678
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Protein Length
Full Length
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
LGP2 (Laboratory of Genetics and Physiology 2) is a member of the RIG-I-like receptor (RLR) family, which plays a crucial role in the innate immune response against viral infections. It is primarily involved in recognizing viral RNA, thereby initiating signaling pathways that lead to the production of type I interferons and other inflammatory cytokines. Understanding LGP2's function and its interactions with viral components and other proteins is essential for elucidating the complex mechanisms of antiviral immunity. Recent studies have highlighted its dual role in both acting as a positive regulator of immune signaling and modulating the activity of other RLRs, such as RIG-I and MDA5. Researchers have focused on the structural characteristics of LGP2, including its RNA-binding domain and ATPase activity, to develop recombinant proteins that can be used in functional assays. These recombinant LGP2 proteins provide valuable insights into its binding affinity for viral RNAs, its role in immune signaling cascades, and its potential as a therapeutic target. Furthermore, investigations into LGP2's expression patterns in different tissues and its regulation upon viral infection contribute to our understanding of host-pathogen interactions. Overall, the study of LGP2 and its recombinant proteins is pivotal for advancing our knowledge of innate immunity and developing novel antiviral strategies.
