Analytical Data
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Gene name
DNMT2
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简介
The DNMT2 protein exhibits remarkable specificity by selectively methylating cytosine 38 within the anticodon loop of tRNA (Asp). This site-specific methylation is critical for epigenetic modification of transfer RNA, affecting its structure, stability, and functional interactions. DNMT2 Protein, Human (sf9, GST) is the recombinant human-derived DNMT2 protein, expressed by Sf9 insect cells , with N-GST labeled tag.
- Application
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Alternative Names
tRNA (cytosine(38)-C(5))-methyltransferase; M.HsaIIP; PuMet; TRDMT1; DNMT2
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Species
Human
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Source
Baculovirus
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Tag
N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O14717
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Expression Region
M1-E391
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Protein Length
Full Length
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Molecular Weight
60 kDa(Predicted Molecular Mass :70.88 kDa)
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DNMT2, or DNA methyltransferase 2, is a member of the DNA methyltransferase family, primarily known for its role in the methylation of cytosine residues in DNA, which is a crucial mechanism in the regulation of gene expression and maintenance of genomic stability. Unlike its better-characterized relatives, DNMT1 and DNMT3, DNMT2 has been shown to primarily methylate RNA rather than DNA, suggesting a unique function in post-transcriptional modifications. The study of DNMT2 recombinant proteins has gained attention due to its potential implications in various biological processes, including RNA metabolism, stress responses, and developmental regulation. Research indicates that DNMT2 may play a role in the defense against retrotransposons, suggesting a protective mechanism in maintaining genome integrity. Furthermore, alterations in DNMT2 expression and function have been implicated in various diseases, including cancer, making it a target for therapeutic intervention. Understanding the structure and function of DNMT2 recombinant proteins can offer insights into their enzymatic activity and substrate specificity, thus paving the way for novel biomolecular applications and drug development strategies aimed at modulating DNA and RNA methylation processes in clinical settings.
