Analytical Data
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Gene name
MEK1
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简介
The MEK1 protein is a dual-specificity kinase that plays a crucial role in the MAP kinase pathway.MEK1 regulates various cellular processes such as growth, adhesion, survival, and differentiation, as well as transcription, metabolism, and cytoskeletal rearrangements.MEK1 Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived MEK1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
- Application
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Alternative Names
Dual specificity mitogen-activated protein kinase kinase 1; MAPKK 1; MEK 1; Map2k1
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Species
Mouse
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Source
Baculovirus
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Tag
N-His;N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P31938
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Expression Region
M1-I393
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Protein Length
Full Length
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Molecular Weight
65 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MEK1 (Mitogen-Activated Protein Kinase Kinase 1) is a pivotal component of the MAPK/ERK signaling pathway, which is crucial for various cellular processes, including proliferation, differentiation, and survival. Dysregulation of this pathway is frequently implicated in numerous diseases, particularly cancer, as it can lead to uncontrolled cell growth and resistance to apoptosis. The study of MEK1 and its associated signaling mechanisms has garnered significant attention in biomedical research, particularly in the context of targeted therapies for malignancies. Recombinant MEK1 proteins are increasingly being utilized in research to elucidate their biochemical properties, understand their interaction with other signaling molecules, and investigate the impact of mutations found in various cancers. By expressing MEK1 in a recombinant system, researchers can produce these proteins in adequate quantities for functional assays, structural studies, and drug screening. The characterization of MEK1's structural and functional properties is essential for the development of inhibitors that can modulate its activity, presenting potential therapeutic avenues for patients with MAPK pathway-related diseases. Overall, the study of recombinant MEK1 proteins plays a vital role in advancing our understanding of cellular signaling networks and developing novel cancer therapies.
