Analytical Data
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Gene name
PLD4
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简介
PLD4 Protein, with 5'->3' DNA exonuclease activity, efficiently digests single-stranded DNA (ssDNA). It crucially regulates inflammatory cytokine responses by degrading nucleic acids, reducing ssDNA concentrations activating TLR9. Additionally, PLD4 actively participates in the phagocytosis process of activated microglia, emphasizing its role in immune responses. PLD4 Protein, Mouse (HEK293, His) is the recombinant mouse-derived PLD4 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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Alternative Names
5'-3' exonuclease PLD4; Choline phosphatase 4; Phospholipase D4; PLD 4
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Species
Mouse
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Source
HEK293
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Tag
C-8*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8BG07-1
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Expression Region
Q58-W503
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Protein Length
Lumenal Domain
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Molecular Weight
60-80 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PLD4 (Phospholipase D4) is a member of the phospholipase D family, which plays a crucial role in various cellular processes, including membrane trafficking, cell signaling, and lipid metabolism. Recent studies have highlighted PLD4's involvement in immune response and inflammation, making it a potential target for therapeutic interventions in diseases characterized by dysregulated immune functions. The enzyme is known for its ability to hydrolyze phosphatidylcholine, generating phosphatidic acid, a lipid signaling molecule that regulates key cellular processes such as cell growth and motility. Moreover, PLD4 has been implicated in the pathogenesis of several conditions, including cancer and neurodegenerative diseases. Researchers are actively exploring the structural properties of PLD4 and characterizing its enzymatic functions through recombinant protein expression and purification. Understanding the functional mechanisms of PLD4 at the molecular level can provide insights into its biological significance and pave the way for the development of novel therapeutic strategies targeting PLD4-related pathways. Consequently, the study of PLD4 recombinant protein is vital for elucidating its role in health and disease and for assessing its potential as a biomarker or therapeutic target in various clinical contexts.
