Analytical Data
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基因名
Wilms tumor protein/WT1
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简介
Wilms tumor protein (WT1) is a key transcription factor that critically regulates cell development and survival by binding to 5'-GCG(T/G)GGGCG-3'. It oversees target genes such as erythropoietin (EPO) and is critical for the development of the genitourinary system. Wilms tumor protein/WT1 Protein, Human (His-SUMO) is the recombinant human-derived Wilms tumor protein/WT1 protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag.
- Application
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别名
WIT 2; WT 1; GUD; WT33
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种属
Human
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表达系统
E. coli
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标签
N-SUMO;N-6*His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
P19544
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表达区间
M1-L449
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氨基酸序列
MGSDVRDLNALLPAVPSLGGGGGCALPVSGAAQWAPVLDFAPPGASAYGSLGGPAPPPAPPPPPPPPPHSFIKQEPSWGGAEPHEEQCLSAFTVHFSGQFTGTAGACRYGPFGPPPPSQASSGQARMFPNAPYLPSCLESQPAIRNQGYSTVTFDGTPSYGHTPSHHAAQFPNHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQLECMTWNQMNLGATLKGVAAGSSSSVKWTEGQSNHSTGYESDNHTTPILCGAQYRIHTHGVFRGIQDVRRVPGVAPTLVRSASETSEKRPFMCAYPGCNKRYFKLSHLQMHSRKHTGEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGKTSEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTKLQLAL
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蛋白长度
Full Length
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分子量
65.2kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Wilms tumor protein (WT1) is a crucial transcription factor initially identified in the context of Wilms tumor, a pediatric kidney cancer. Its gene, located on chromosome 11p13, plays a significant role in kidney and gonadal development. Mutations or abnormal expression of WT1 are linked to various syndromes, including WAGR syndrome and Denys-Drash syndrome, both associated with increased risk of Wilms tumor and other developmental disorders. The study of WT1 has expanded beyond oncology to include its involvement in stem cell biology, hematopoiesis, and organogenesis. Recombinant WT1 protein is instrumental in elucidating its functional properties and regulatory mechanisms, providing insights into its role in tumorigenesis and normal development. Researchers utilize recombinant WT1 in various applications, including immunotherapy, where it is explored as a target for vaccines against Wilms tumor and other cancers. Understanding the precise interactions and pathways mediated by WT1 could lead to novel therapeutic strategies and improve clinical outcomes for patients with malignancies associated with WT1 dysregulation. Thus, the production and study of recombinant WT1 protein continue to be a priority in cancer research, emphasizing its potential as both a biomarker and therapeutic target.
