Analytical Data
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基因名
CAPN1
- Application
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别名
PIG30; CANPL1; MuCANP; MuCL; Calpain 1, Large Subunit; Calpain-1 Catalytic Subunit; Calcium-Dependent Protease 1; Calcium-activated neutral proteinase 1; Calpain mu-type; Micromolar-calpain
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种属
Rat
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表达系统
E. coli
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标签
N-His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
P97571
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表达区间
Pro75~Asp356
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分子量
33kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CAPN1, or calpain-1, is a calcium-dependent cysteine protease that plays a crucial role in various cellular processes, including cell signaling, apoptosis, and cytoskeletal remodeling. Dysregulation of CAPN1 has been implicated in several pathological conditions, including neurodegenerative diseases, cardiovascular disorders, and cancer. The study of CAPN1 and its functions has garnered increasing interest due to its potential as a therapeutic target. Recombinant CAPN1 protein production allows for detailed biochemical studies and structural analyses, facilitating the understanding of its enzymatic mechanisms and interactions with substrates. By utilizing techniques such as molecular cloning and expression systems, researchers can generate large quantities of this enzyme, enabling in vitro assays to explore its role in cellular pathways and disease mechanisms. Furthermore, recombinant CAPN1 can be employed in drug screening assays to identify small molecules that modulate its activity, thereby offering insights into novel treatment strategies for related diseases. Overall, the study of CAPN1 through recombinant protein techniques is pivotal, not only for elucidating its biological functions but also for advancing therapeutic interventions targeting calpain-related pathways.












